FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_8_NLP7_bio_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_8_NLP7_bio_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12796183
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC153141011.967709433352118TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1815041.4184229781646605TruSeq Adapter, Index 8 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG818180.6393937942275443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC272110.2126493502007591TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC211010.16490073641491373TruSeq Adapter, Index 8 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC150220.11739438237168068TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC132060.1032026503528435TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC129440.10115516478624915TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG1904250.066.3979970
TCTCGTA1973200.059.97327841
CTCGTAT1977500.059.92582342
TCGTATG2004800.059.73826643
CGTATGC2030300.059.600444
TATGCCG2029900.059.5428546
GCCGTCT1971850.059.50702749
CCGTCTT1989750.059.46186450
ATCTCGT1996850.059.37247540
GTATGCC2049150.059.29638345
ATGCCGT2025050.059.17259247
AATCTCG2004900.059.01537739
AAAAAGG2149750.058.94936869
CGTCTTC1986900.058.93504351
TGCCGTC2025150.058.67491548
CTGCTTG1962000.058.54468557
TGCTTGA1982050.058.49816558
GTCTTCT1981000.058.37447452
TTCTGCT1973200.058.21661455
CTTCTGC2005550.058.19600354