Basic Statistics
Measure | Value |
---|---|
Filename | H2YNTBGXB_n01_8_NLP7_bio_50.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12796183 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC | 1531410 | 11.967709433352118 | TruSeq Adapter, Index 8 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG | 181504 | 1.4184229781646605 | TruSeq Adapter, Index 8 (100% over 49bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 81818 | 0.6393937942275443 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC | 27211 | 0.2126493502007591 | TruSeq Adapter, Index 8 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC | 21101 | 0.16490073641491373 | TruSeq Adapter, Index 8 (98% over 50bp) |
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC | 15022 | 0.11739438237168068 | TruSeq Adapter, Index 8 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC | 13206 | 0.1032026503528435 | TruSeq Adapter, Index 8 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC | 12944 | 0.10115516478624915 | TruSeq Adapter, Index 8 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAAGGG | 190425 | 0.0 | 66.39799 | 70 |
TCTCGTA | 197320 | 0.0 | 59.973278 | 41 |
CTCGTAT | 197750 | 0.0 | 59.925823 | 42 |
TCGTATG | 200480 | 0.0 | 59.738266 | 43 |
CGTATGC | 203030 | 0.0 | 59.6004 | 44 |
TATGCCG | 202990 | 0.0 | 59.54285 | 46 |
GCCGTCT | 197185 | 0.0 | 59.507027 | 49 |
CCGTCTT | 198975 | 0.0 | 59.461864 | 50 |
ATCTCGT | 199685 | 0.0 | 59.372475 | 40 |
GTATGCC | 204915 | 0.0 | 59.296383 | 45 |
ATGCCGT | 202505 | 0.0 | 59.172592 | 47 |
AATCTCG | 200490 | 0.0 | 59.015377 | 39 |
AAAAAGG | 214975 | 0.0 | 58.949368 | 69 |
CGTCTTC | 198690 | 0.0 | 58.935043 | 51 |
TGCCGTC | 202515 | 0.0 | 58.674915 | 48 |
CTGCTTG | 196200 | 0.0 | 58.544685 | 57 |
TGCTTGA | 198205 | 0.0 | 58.498165 | 58 |
GTCTTCT | 198100 | 0.0 | 58.374474 | 52 |
TTCTGCT | 197320 | 0.0 | 58.216614 | 55 |
CTTCTGC | 200555 | 0.0 | 58.196003 | 54 |