FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_7_NLP7_bio_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_7_NLP7_bio_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10443837
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC181309017.360382012856004TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2173782.081399776729568TruSeq Adapter, Index 7 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG785900.7525012119587848No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC191090.18296915204632166TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC182260.17451440500268245TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC139660.1337247986539813TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC129910.12438914931360956TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT121760.11658550396755522TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATAATCTCGTATGC104910.10045158690239996TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2154150.067.5251370
AAAAAGG2434750.059.93477669
TCTCGTA2351300.059.69789541
CTCGTAT2376300.059.51639642
AAAAAAG2486500.059.4458668
TCGTATG2403750.059.3416643
CGTATGC2428200.059.2549644
TATGCCG2425800.059.1736346
ATCTCGT2371500.059.152540
GCCGTCT2395350.059.14087349
GTATGCC2431500.059.13510545
TGCTTGA2379600.059.11209558
CCGTCTT2399850.059.1078450
GCTTGAA2398700.059.0237359
TTCTGCT2380400.058.83014355
ATGCCGT2430750.058.82446747
CTTGAAA2422200.058.80280760
CATCTCG2366150.058.68782439
CTTCTGC2403800.058.68707754
ATCATCT2352650.058.60246737
Produced by FastQC (version 0.11.5)