FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_6_NLP7_bio_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_6_NLP7_bio_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10807869
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC246071822.767837026892167TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4475284.140760773469775TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1330951.231463852864982No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC299900.27748300798242465TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT188800.17468753553545108TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC154030.1425165312421903TruSeq Adapter, Index 6 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG118470.10961457804494115TruSeq Adapter, Index 6 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG112390.1039890472395622No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGATCTCGTATGC109540.10135207967454084TruSeq Adapter, Index 6 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2956950.067.41296470
AAAAAGG3316350.060.18643669
AAAAAAG3398850.059.2910768
CTCGTAT3375800.056.7195142
TCGTATG3406150.056.67292843
TCTCGTA3375350.056.6656341
TATGCCG3395200.056.65416346
CGTATGC3403550.056.64861344
GCCGTCT3388250.056.5792749
GTATGCC3402150.056.56414845
CCGTCTT3388100.056.547750
ATGCCGT3403000.056.45668847
ATCTCGT3395750.056.3396440
TATCTCG3349450.056.33263839
CGTCTTC3402950.056.30249851
TGCCGTC3408550.056.2928848
TGCTTGA3404100.056.23954858
GCTTGAA3410950.056.22435859
CTTGAAA3421550.056.20311760
CTGCTTG3402800.056.1540557
Produced by FastQC (version 0.11.5)