FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_5_NLP7_bio_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_5_NLP7_bio_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53598299
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10793922.0138549546133917TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1382350.257909304174TruSeq Adapter, Index 5 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG944140.1761511125567623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1492900.057.90479346
CGTATGC1497250.057.89524544
GCCGTCT1481050.057.64807549
GTATGCC1505750.057.53096445
AAAAGGG1548150.057.5194170
TCTCGTA1451950.057.22025341
CTCGTAT1489450.056.48727442
ATCTCGT1455450.056.38780240
CCGTCTT1527700.055.9532950
TCGTATG1543750.055.90149743
ATGCCGT1541500.055.88903447
GTCACAC1547700.055.87885729
ACGTCTG1599200.055.62469515
GCACACG1605400.055.4306311
CGTCTGA1609750.055.2645916
CACACGT1611300.055.2535212
ACACGTC1615700.055.1179513
AGCACAC1647950.054.06574610
TGCTTGA1587100.053.47012358
CAGTCAC1653100.053.4575527
Produced by FastQC (version 0.11.5)