FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_4_BZIP1_bio_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_4_BZIP1_bio_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8018897
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC280687435.00324296471198TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG4200545.23830147712335TruSeq Adapter, Index 4 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1541281.9220598543665046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC743270.92689805094142TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATGC448030.5587177388610928TruSeq Adapter, Index 4 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC284660.3549864775666778TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG170490.21261028792363837TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGC165300.20613807609699938TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT159390.1987679851730232TruSeq Adapter, Index 4 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT156020.1945654121757643Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG138810.17310360764080146Illumina Single End PCR Primer 1 (96% over 32bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG121250.1512053341001886TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACAAATCTCGTATGC120700.15051945423416713TruSeq Adapter, Index 4 (98% over 50bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG103940.12961882413504003TruSeq Adapter, Index 4 (97% over 47bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT103340.1288705915539257Illumina Single End PCR Primer 1 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATG100570.12541625113778118TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATG93570.11668687102478058TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTCTCGTATGC84180.10497703113034125TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCCATCTCGTATGC82450.10281962718812823TruSeq Adapter, Index 4 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3399800.068.21586670
AAAAAGG3769250.061.60013669
AAAAAAG3824650.061.2708168
TCTCGTA3730500.058.22496441
ATCTCGT3719300.058.1349340
CTCGTAT3768450.058.07828542
AATCTCG3689800.057.96806339
TGCTTGA3873700.057.84202658
GCTTGAA3877400.057.83016259
CTTGAAA3887450.057.80660
TCGTATG3906700.057.7804343
TATGCCG3898350.057.77993846
TTCTGCT3877250.057.7771555
GCCGTCT3883650.057.77080549
CCGTCTT3882100.057.7700750
CAATCTC3672250.057.75150338
CGTATGC3906850.057.74596444
CCAATCT3653800.057.72894737
GTATGCC3902300.057.725445
ACCAATC3595000.057.7253236
Produced by FastQC (version 0.11.5)