FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_3_BZIP1_bio_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_3_BZIP1_bio_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11126213
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC216011119.414611242837072TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3490833.137482627736859TruSeq Adapter, Index 3 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1200911.0793519771731854No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC301990.27142209123625444TruSeq Adapter, Index 3 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC219130.19694931240306113TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCGTATGC200700.1803848263555623TruSeq Adapter, Index 3 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATG181470.16310131758218183TruSeq Adapter, Index 3 (97% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT153270.1377557664948532TruSeq Adapter, Index 3 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG152830.13736030399561827Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT128070.11510655062958079Illumina Single End PCR Primer 1 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2620900.067.5863170
AAAAAGG2965100.059.83669
AAAAAAG3039350.058.992868
TCTCGTA2880250.057.60261541
ATCTCGT2887200.057.3112140
CTCGTAT2936900.057.27583742
TCGTATG2990350.057.00724843
CGTATGC3001700.056.9472844
CATCTCG2871700.056.93386539
GCATCTC2859550.056.9187438
GCCGTCT2977300.056.90875249
TATGCCG3000450.056.90010546
CCGTCTT2980400.056.885350
GTATGCC3007450.056.8250945
TGCTTGA2968500.056.8250258
ATGCCGT3002150.056.71982247
TTCTGCT2966350.056.71475255
GCTTGAA2988650.056.6585659
CTTGAAA3001850.056.64678660
TGCCGTC3005700.056.51775748
Produced by FastQC (version 0.11.5)