FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_2_BZIP1_bio_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_2_BZIP1_bio_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10501415
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC504416348.03317457694987TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG6884226.555516566100854TruSeq Adapter, Index 2 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2388022.2739983135606012No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC584580.5566678395244831TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC413100.3933755593889014TruSeq Adapter, Index 2 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT342480.32612747901116185Illumina Single End PCR Primer 1 (96% over 30bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT324460.3089678867086007TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC314180.2991787297235658TruSeq Adapter, Index 2 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG278090.2648119324871934Illumina Single End PCR Primer 1 (96% over 32bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG276060.2628788596584365TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC249610.23769177772709676TruSeq Adapter, Index 2 (98% over 50bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG236070.22479827718455084TruSeq Adapter, Index 2 (97% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC222980.21233329032325643TruSeq Adapter, Index 2 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT208990.19901127609945898Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATGTATCTCGTATGC205080.19528796833569573TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATG182100.17340520301311776TruSeq Adapter, Index 2 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGG154140.1467802196180229No Hit
GATCGGAAAAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC153370.14604698509677028TruSeq Adapter, Index 2 (98% over 50bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC146280.13929551398549625TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG145750.13879082009424445TruSeq Adapter, Index 2 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGTATCTCGTATGC136730.13020150141671386TruSeq Adapter, Index 2 (98% over 50bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG135380.12891596037295927TruSeq Adapter, Index 2 (100% over 47bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG123740.11783173981791978No Hit
GATAGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC120610.11485118910165915TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG119310.11361326068915474TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC113970.10852823167163664TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAACACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC112950.10755693399413317TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC111720.106385663265379TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTATGC111070.10576669905912679TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG108050.10289089613161656Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG5925500.068.3364770
AAAAAGG6552250.061.88023469
AAAAAAG6643600.061.6369368
TTCTGCT6555100.058.93268655
TGCTTGA6573750.058.9284558
TCTCGTA6586850.058.90661241
CTGCTTG6558350.058.83915357
TCTGCTT6590650.058.81396556
CTTCTGC6602500.058.8005954
TCTTCTG6590200.058.8001453
CTCGTAT6637300.058.7900242
GCTTGAA6629050.058.7703359
CTTGAAA6668000.058.71687360
GCCGTCT6647950.058.70993849
GTCTTCT6613800.058.68845452
CCGTCTT6662050.058.6860650
TCGTATG6703450.058.6855243
ATCTCGT6621100.058.635240
TATGCCG6726650.058.58648746
TTGAAAA6710850.058.5809561
Produced by FastQC (version 0.11.5)