FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_20_NLP7_ab_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_20_NLP7_ab_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5956388
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT5116828.590474629926726TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA846741.4215662243628187TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475020.7974967379559559No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG61710.10360305608029564TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA682200.056.6698243
TATCTCG678300.056.66542441
TTATCTC675900.056.5611240
ATCTCGT682750.056.4545342
CGTATGC706450.056.21623646
GTATGCC705900.056.1573347
GCTTGAA691600.056.1422761
TATGCCG703600.056.1364848
TGCTTGA687000.056.1285860
GCCGTCT697300.056.11443751
CTCGTAT692700.056.0841244
CCGTCTT699950.056.02653552
GGCCTTA703000.055.91366236
CTTATCT685000.055.8659339
TCACGTG725750.055.7842430
GTCACGT731250.055.77147329
TCGTATG705100.055.768445
CACGTGG725200.055.6624631
TGAAAAA726100.055.6200764
TGAACTC757400.055.493220
Produced by FastQC (version 0.11.5)