FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_1_BZIP1_bio_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_1_BZIP1_bio_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12338090
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC262045721.23875737654694TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3975663.2222653587386705TruSeq Adapter, Index 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1391861.1281000543844306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC342400.27751459099423004TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC323350.2620745998772906TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC290060.2350931140881611TruSeq Adapter, Index 1 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC273560.22171989343569387TruSeq Adapter, Index 1 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT206140.1670761033514912Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATG183200.14848327415345489TruSeq Adapter, Index 1 (97% over 49bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG173340.14049176169082897TruSeq Adapter, Index 1 (97% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG172700.1399730428291575Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATG165740.13433197520848042TruSeq Adapter, Index 1 (97% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT161670.13103324744753847TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC145750.11813011576346097TruSeq Adapter, Index 1 (98% over 50bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG143930.11665500900058276TruSeq Adapter, Index 1 (97% over 47bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT134060.10865539155574322Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3235600.067.8456770
AAAAAAG3739900.059.40751668
AAAAAGG3726400.059.03166669
ATCTCGT3539900.057.58590740
TCTCGTA3586550.057.5687741
CTCGTAT3660850.057.15537342
TGCTTGA3719250.057.0605158
GCTTGAA3723500.057.03533659
CGATCTC3529250.057.01429738
GCCGTCT3728300.056.94933349
CTTGAAA3738700.056.9476560
TATGCCG3742150.056.9272646
CGTATGC3750650.056.92678544
GTATGCC3744450.056.92313445
CCGTCTT3730050.056.8808450
ATGCCGT3741450.056.8794347
TCGTATG3758600.056.83268443
TGCCGTC3740950.056.8000348
TTCTGCT3738100.056.7617855
TGAAAAA3783300.056.6132862
Produced by FastQC (version 0.11.5)