FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_19_2XNLP7_novus_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_19_2XNLP7_novus_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272897
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT35915815.801771923672739TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA521352.293768701353383TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208810.9186953918281383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT156590.6889445496210342TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT49830.21923562748333955TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG44770.19697329003470018TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTAT41540.18276235130760435TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT40890.17990256487645503TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTA39060.17185116615491156TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT32730.1440012459869497TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTA28560.12565461611326867TruSeq Adapter, Index 19 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCC24540.10796793695446824TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAC1050.059.99865728
CGATCTC461450.058.19046840
ATCTCGT468400.058.1776142
TCTCGTA493250.058.07067543
TTCCCTA10050.057.8083848
GATCTCG468250.057.5982941
ACGATCT465950.057.5883939
CTCGTAT504350.057.24368744
GCTTGAA516800.057.2186461
TGCTTGA513850.057.1179860
TGAAAAA535700.057.06991264
AAACGAT487450.057.0574537
CGTATGC525900.057.0288846
TATGCCG523250.056.9966448
GTATGCC524800.056.9950347
CCGTCTT523100.056.9151252
GCCGTCT522500.056.90553751
CGTCTGA560000.056.76998516
ACGTCTG560200.056.76221515
TGAACTC558300.056.7372420
Produced by FastQC (version 0.11.5)