FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_18_BZIP1_ab_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_18_BZIP1_ab_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16370399
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT4975113.0390890289234855TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA676390.41317868916939654TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG508350.3105299999102038No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACCTCTCGTAT183210.11191541513435317TruSeq Adapter, Index 18 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA686200.057.49815443
TATGCCG704650.057.3882148
CGTATGC706550.057.38762346
GCCGTCT698850.057.34609651
GTATGCC708050.057.23109447
ATGCCGT706500.057.2181249
ATCTCGT665300.057.1206942
TGCCGTC706350.057.0630250
CATCTCG662400.056.96391741
CCGTCTT706600.056.91152652
GCTTGAA703450.056.5964161
TGCTTGA695650.056.57652760
CACATCT673000.056.1495839
CTTGAAA719300.055.7884562
TCCGCAC747800.054.37832635
CGCACAT719550.053.81446537
CTCGTAT738700.053.68651644
CTGCTTG731100.053.55634359
TCGTATG752750.053.4788945
CGTCCGC770800.053.2673333
Produced by FastQC (version 0.11.5)