FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_17_BZIP1_ab_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_17_BZIP1_ab_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4081900
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC3728669.134618682476297TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308400.7555305127514148No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG269820.6610157034714227TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC121570.2978269923320022TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGCTCTCGTATGC46860.11479948063401847TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG465450.064.5019270
TCTCGTA481550.061.32674441
CGTATGC489750.061.20261444
TATGCCG490000.061.15070746
GCCGTCT488800.061.14404749
GTATGCC490200.061.1107345
ATCTCGT477400.061.09816440
ATGCCGT490950.061.0117447
CCGTCTT489550.060.94388250
TGCCGTC490800.060.93129348
GCTTGAA490550.060.85191359
TGCTTGA491200.060.73575258
CTTGAAA493950.060.5542660
CTCGTAT494250.060.2693242
TCGTATG499750.060.07599643
CTGCTTG498100.059.8093457
AGAGATC473000.059.64575636
ACGTAGA482900.059.6108332
TCTGCTT500750.059.50899556
GTCACGT492450.059.44121629