FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_16_BZIP1_novus_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_16_BZIP1_novus_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20642532
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT5180482.509614615106325TruSeq Adapter, Index 16 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTA565640.27401677274861436TruSeq Adapter, Index 16 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422200.2045291730684976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC726400.058.52657346
TCTCGTA693850.058.52208743
TATGCCG723900.058.50287648
ATGCCGT725400.058.42078449
GTATGCC729750.058.3738547
GCCGTCT711950.058.21845651
ATCTCGT674500.058.17703642
TGCCGTC723500.058.12970450
CATCTCG672650.058.1287741
GCATCTC675000.057.94265440
TCCGCAT708300.057.8269737
CGTCCGC749900.057.7034635
CCGTCTT722650.057.5507452
CCGTCCG756300.057.43700434
CCCGTCC759250.057.29653533
TGCTTGA699300.057.15689560
GCTTGAA710500.057.138361
CACCCGT772700.056.75281531
ACCCGTC770900.056.60344732
TCACCCG778050.056.55218530
Produced by FastQC (version 0.11.5)