FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_15_BZIP1_novus_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_15_BZIP1_novus_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19907394
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT9594934.819782036764832TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA1283540.6447554109794582TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639320.3211470069864494No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT292620.14699061062437405TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1262350.057.24804743
CGTATGC1292850.057.1671546
TATGCCG1283400.057.1326648
GTATGCC1304800.057.07530247
ATGCCGT1286150.057.02668449
GCCGTCT1243600.056.84404851
TGCCGTC1278450.056.7276650
ATCTCGT1265350.056.69727742
AATCTCG1274050.056.5433341
CCGTCTT1272950.056.2714352
TGCTTGA1191050.055.50603560
GCTTGAA1222600.055.476661
ATGTCAG1455700.055.34556634
CTCGTAT1304300.055.34520344
GTCACAT1489900.055.2528329
ACATGTC1470000.055.06081432
TCGTATG1323350.054.9237845
CTTGAAA1279150.054.22553362
CTGCTTG1198900.054.14755259
AGAATCT1368450.053.61777539
Produced by FastQC (version 0.11.5)