FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_14_NLP7_ab_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_14_NLP7_ab_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18584930
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT15898348.554425548011212TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA1555900.8371836751604661TruSeq Adapter, Index 14 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1147380.6173711711585677No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG453080.24378891930182142TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT208420.11214462470399406TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2028650.059.36049343
CGTATCT2017800.059.2648139
CGTATGC2082800.059.20783646
TATGCCG2076450.059.17683848
GTATGCC2085450.059.16832447
ATCTCGT2023750.059.16525342
GCCGTCT2050850.059.1173551
CCGTCTT2064100.058.9065352
TGCTTGA2007300.058.83901660
GCTTGAA2030800.058.825261
TATCTCG2033250.058.73357841
CTCGTAT2072800.058.35678544
CCGTATC2078800.058.3254738
TCCGTAT2101400.058.2746237
GTTCCGT2131450.058.25239635
TTCCGTA2111400.058.1842736
TCGTATG2101750.058.11264845
AGTTCCG2142750.058.08535434
CACAGTT2191600.058.03214331
TGAACTC2290750.057.6888720
Produced by FastQC (version 0.11.5)