FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_13_NLP7_ab_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_13_NLP7_ab_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18590394
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT9117194.904247860481063TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA992200.5337164989617756TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG376610.20258311900221157No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACACTCTCGTAT236240.12707638149035463TruSeq Adapter, Index 13 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1247450.058.41616446
TATGCCG1240650.058.4134848
GTATGCC1245800.058.3274647
TCTCGTA1213850.058.2899943
GCCGTCT1238000.058.16422351
ACGTCTG1347650.057.8643715
ATCTCGT1193550.057.86195442
GCACACG1350750.057.82883511
CACACGT1353800.057.69322212
CGTCTGA1352700.057.6462316
ACACGTC1354750.057.63985413
CCGTCTT1257650.057.41552
ACAGTCA1286100.057.3992732
CTCGTAT1236000.057.30799544
AGCACAC1367500.057.17952310
ACAATCT1200000.057.01551439
TGAACTC1364250.057.0002120
GAGCACA1371350.056.9639
ATGCCGT1276650.056.84060749
TCGTATG1274350.056.72867245
Produced by FastQC (version 0.11.5)