FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_12_NLP7_novus_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_12_NLP7_novus_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20413709
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC217125110.636239597615504TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2539461.243997354914778TruSeq Adapter, Index 12 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG869240.42581188945134857No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC273090.13377774710122498TruSeq Adapter, Index 12 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC204880.10036392700611142TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2635800.066.6306370
TCTCGTA2844750.059.08843241
CGTATGC2911900.059.0195544
TATGCCG2906450.058.99462546
GTATGCC2911150.058.95347645
GCCGTCT2893550.058.95290849
CTCGTAT2867900.058.90004742
ATCTCGT2848700.058.8833540
AAAAAAG3015600.058.87508868
TCGTATG2932250.058.66485643
TGCTTGA2908150.058.6124358
CCGTCTT2914850.058.5588650
TGTAATC2810150.058.48935336
GCTTGAA2922350.058.47179459
AAAAAGG3011500.058.44728569
TAATCTC2845100.058.1635538
AATCTCG2870450.058.08057839
GTAATCT2843100.057.99462537
ATGCCGT2967600.057.7076147
TGAAAAA2998050.057.68931262
Produced by FastQC (version 0.11.5)