FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_11_NLP7_novus_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_11_NLP7_novus_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13103646
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC337852825.783114104272965TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG2842162.168984113276564TruSeq Adapter, Index 11 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1340331.0228679865130665No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG329030.2510980531677977TruSeq Adapter, Index 11 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC317190.24206240003736365TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATGC313480.23923112697031038TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGC275980.21061313774807408TruSeq Adapter, Index 11 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT231850.1769354880313464Illumina Single End PCR Primer 1 (96% over 30bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG214490.16368726688739912TruSeq Adapter, Index 11 (100% over 47bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG212970.16252728439092448TruSeq Adapter, Index 11 (97% over 47bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGT187510.14309757757497418TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACCTCTCGTATGC180620.13783949902187528TruSeq Adapter, Index 11 (98% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG178140.13594689600131138Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3931150.067.84598570
AAAAAAG4316550.062.41348368
AAAAAGG4291800.062.22350769
TCTCGTA4264650.061.1632241
GATCGGA4394700.061.160071
TGCTTGA4327550.061.05128558
GCTTGAA4337250.061.02958359
GCCGTCT4337550.061.02624549
ATCTCGT4248550.061.01740
TATGCCG4356100.061.00636746
GTATGCC4359750.060.9794845
CGTATGC4367800.060.9771144
CCGTCTT4341400.060.9486450
CTCGTAT4323650.060.9095842
CATCTCG4213850.060.90957639
TCGTATG4381500.060.80309743
CTTGAAA4368050.060.75826660
TGAAAAA4401050.060.75461662
ACATCTC4222450.060.55462638
ATGCCGT4385900.060.5125447
Produced by FastQC (version 0.11.5)