FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_10_input_NLP7_BIO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_10_input_NLP7_BIO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827468
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC204834713.814543386638906TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG2359341.5911954758560263TruSeq Adapter, Index 10 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG938910.6332234202090337No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC352930.2380244556926375TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCGTATGC211120.14238439091556293TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC192280.12967824310934276TruSeq Adapter, Index 10 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG177630.11979793178444223TruSeq Adapter, Index 10 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG2475800.067.01932570
TCTCGTA2656200.059.4242341
GCCGTCT2644250.059.2572749
TGCTTGA2621400.059.22789458
CGTATGC2708600.059.14204844
CTCGTAT2678500.059.128142
TATGCCG2708600.059.10706746
GTATGCC2718250.059.09670345
CCGTCTT2668500.059.09449450
ATCTCGT2681950.059.05615640
GCTTGAA2660600.059.0006359
TCGTATG2699700.058.98309743
ATGCCGT2706650.058.79961447
TATCTCG2688300.058.72318339
TTCTGCT2642850.058.43943855
CTTGAAA2707400.058.4216160
CTTCTGC2670200.058.41556554
AAAAAAG2876500.058.40408768
TGCCGTC2715350.058.1985548
TGAAAAA2812250.057.86707362