Basic Statistics
Measure | Value |
---|---|
Filename | H2YNTBGXB_n01_10_input_NLP7_BIO.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14827468 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC | 2048347 | 13.814543386638906 | TruSeq Adapter, Index 10 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG | 235934 | 1.5911954758560263 | TruSeq Adapter, Index 10 (100% over 49bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 93891 | 0.6332234202090337 | No Hit |
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC | 35293 | 0.2380244556926375 | TruSeq Adapter, Index 10 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCGTATGC | 21112 | 0.14238439091556293 | TruSeq Adapter, Index 10 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC | 19228 | 0.12967824310934276 | TruSeq Adapter, Index 10 (98% over 50bp) |
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG | 17763 | 0.11979793178444223 | TruSeq Adapter, Index 10 (97% over 49bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAAAGGG | 247580 | 0.0 | 67.019325 | 70 |
TCTCGTA | 265620 | 0.0 | 59.42423 | 41 |
GCCGTCT | 264425 | 0.0 | 59.25727 | 49 |
TGCTTGA | 262140 | 0.0 | 59.227894 | 58 |
CGTATGC | 270860 | 0.0 | 59.142048 | 44 |
CTCGTAT | 267850 | 0.0 | 59.1281 | 42 |
TATGCCG | 270860 | 0.0 | 59.107067 | 46 |
GTATGCC | 271825 | 0.0 | 59.096703 | 45 |
CCGTCTT | 266850 | 0.0 | 59.094494 | 50 |
ATCTCGT | 268195 | 0.0 | 59.056156 | 40 |
GCTTGAA | 266060 | 0.0 | 59.00063 | 59 |
TCGTATG | 269970 | 0.0 | 58.983097 | 43 |
ATGCCGT | 270665 | 0.0 | 58.799614 | 47 |
TATCTCG | 268830 | 0.0 | 58.723183 | 39 |
TTCTGCT | 264285 | 0.0 | 58.439438 | 55 |
CTTGAAA | 270740 | 0.0 | 58.42161 | 60 |
CTTCTGC | 267020 | 0.0 | 58.415565 | 54 |
AAAAAAG | 287650 | 0.0 | 58.404087 | 68 |
TGCCGTC | 271535 | 0.0 | 58.19855 | 48 |
TGAAAAA | 281225 | 0.0 | 57.867073 | 62 |