FastQCFastQC Report
Tue 20 Dec 2022
H2Y7MAFX5_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y7MAFX5_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213199937
Sequences flagged as poor quality0
Sequence length90
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA14374010.6742032949099793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8308070.3896844491093822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3364000.1577861629480688Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGTTGAACTCTAGATAA2863230.13429788208614715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2843500.13337245967385067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)