FastQCFastQC Report
Tue 20 Dec 2022
H2Y7MAFX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y7MAFX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213199937
Sequences flagged as poor quality0
Sequence length10
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCACCCTC12808284060.07639673927295No Hit
AGGACGAAAC7529378435.31604420689862No Hit
AGATCTCGGT17272100.8101362619070568Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG4610510.21625287816102873No Hit
TCCCACCCGC3904050.18311684585535312No Hit
TCCCCCCCTC3354290.1573307219129244No Hit
TCCCACCCAC3171460.14875520343141563No Hit
AGGACGAACC2768550.12985698021102135No Hit
AGGAAGAAAC2734110.128241595118295No Hit
TCCCACACTC2669240.12519891129236121No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)