FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_NGFR_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_NGFR_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177154
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT6591218.06051836616994No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3083863.771312121552315No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1309571.6014985164765148No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1284981.571426929222563No Hit
TATTTTTTTTTTTTTTTTTTTTTTT762750.9327817477816854No Hit
TTTTTATTTTTTTTTTTTTTTTTTT660840.8081540350102248No Hit
TTTATTTTTTTTTTTTTTTTTTTTT548270.6704900017781248No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG538110.6580651410013802No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA490050.5992916361854015No Hit
TTATTTTTTTTTTTTTTTTTTTTTT420380.5140908438314846No Hit
TTTTATTTTTTTTTTTTTTTTTTTT388280.47483513212543144No Hit
TTTTTTATTTTTTTTTTTTTTTTTT365980.44756403022371843No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT362400.4431859788870308No Hit
TATTTTTTTTTTTTTTTTTTTTTTA351910.4303575547189156No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT333340.4076479420590587No Hit
TTTTTTTTTTTTTTTTTTTTTTATT309760.3788115033665747No Hit
TTTTTTTATTTTTTTTTTTTTTTTT307320.37582758010916756No Hit
TTTTTATTTTTTTTTTTTTTTTTTA288160.3523964450223146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG265100.32419592440108136No Hit
TTTTTTTTATTTTTTTTTTTTTTTT262010.32041710355461084No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC261390.3196588935465811No Hit
TTTATTTTTTTTTTTTTTTTTTTTA236950.2897707441977979No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC191680.23440918441795275No Hit
TTATTTTTTTTTTTTTTTTTTTTTA190090.23246474262316694No Hit
TTTTTTTTTTTTTTTTTTTTTAATT175780.2149647664700946No Hit
TTTTATTTTTTTTTTTTTTTTTTTA172990.211552821433961No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA167810.20521809910880973No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA162480.19869993887848997No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG156190.19100777605509203No Hit
TTTTTTATTTTTTTTTTTTTTTTTA155840.1905797542763656No Hit
TATTTTTTTTTTTTTTTTTTTTTAA153030.1871433508528762No Hit
TATTTTTTTTTTTTTTTTTTTTTAT144570.17679745300137434No Hit
TTTTTATTTTTTTTTTTTTTTTTAT137100.16766224532398435No Hit
TTTTTTTATTTTTTTTTTTTTTTTA136640.16709970241480104No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC133870.16371221576602324No Hit
TATATTTTTTTTTTTTTTTTTTTTT133630.1634187151177537No Hit
TATTTATTTTTTTTTTTTTTTTTTT131540.16086281363907295No Hit
TTTTTTTTTATTTTTTTTTTTTTTT121410.1484746404433621No Hit
AATTTTTTTTTTTTTTTTTTTTTTT121290.14832789011922728No Hit
TTTATATTTTTTTTTTTTTTTTTTT120390.14722726268821645No Hit
TTTTTATTTTTTTTTTTTTTTTTAA116790.14282475296417313No Hit
TTTTTTTTATTTTTTTTTTTTTTTA111500.13635550950856495No Hit
TTTATTTTTTTTTTTTTTTTTTTAT111340.1361598424097186No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC109830.13431323416435595No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA103410.1264620918231453No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC99720.12194951935600089No Hit
TTTATTTTTTTTTTTTTTTTTTTAA95840.11720459220897637No Hit
TTTTTTTTTTATTTTTTTTTTTTTT95030.11621402752106662No Hit
TTATTATTTTTTTTTTTTTTTTTTT94040.1150033373469547No Hit
TATTATTTTTTTTTTTTTTTTTTTT88670.10843626034192336No Hit
TTATTTTTTTTTTTTTTTTTTTTAT86630.10594150483163214No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT84260.10304318592997026No Hit
TTTTTTTTTTTATTTTTTTTTTTTT82900.1013800155897761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)