FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_NGFR_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_NGFR_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10408079
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT9425169.055619197356208No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4485424.309556066974511No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1950841.874351645486165No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1688621.622412743023953No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1025720.9855036649894758No Hit
TTTTTATTTTTTTTTTTTTTTTTTT880160.845650768023571No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG850280.8169423003034468No Hit
TTTATTTTTTTTTTTTTTTTTTTTT767160.7370812615853511No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA742670.713551463243121No Hit
TTATTTTTTTTTTTTTTTTTTTTTT603220.5795690059616189No Hit
TTTTATTTTTTTTTTTTTTTTTTTT541320.5200959754436913No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT488170.4690298757340332No Hit
TTTTTTATTTTTTTTTTTTTTTTTT486770.46768476680471005No Hit
TATTTTTTTTTTTTTTTTTTTTTTA474250.4556556498081923No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT447500.4299544613371978No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC420170.4036960134526266No Hit
TTTTTTTATTTTTTTTTTTTTTTTT413380.3971722351454096No Hit
TTTTTTTTTTTTTTTTTTTTTTATT392100.3767265794196989No Hit
TTTTTATTTTTTTTTTTTTTTTTTA386370.37122124073039797No Hit
TTTTTTTTATTTTTTTTTTTTTTTT348870.335191537266387No Hit
TTTATTTTTTTTTTTTTTTTTTTTA335030.321894174707936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG314240.3019193071074883No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC294310.28277072070648196No Hit
TTATTTTTTTTTTTTTTTTTTTTTA274820.26404488282612No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG253280.24334942115639205No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA248720.23896820921516831No Hit
TTTTATTTTTTTTTTTTTTTTTTTA237430.2281208664922701No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA234010.2248349575363523No Hit
TTTTTTTTTTTTTTTTTTTTTAATT232730.22360514365811404No Hit
TATTTTTTTTTTTTTTTTTTTTTAA213980.20559029192610856No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC211050.20277517109545382No Hit
TTTTTTATTTTTTTTTTTTTTTTTA207630.19948926213953605No Hit
TTTTTTTATTTTTTTTTTTTTTTTA183070.17589220835083977No Hit
TATATTTTTTTTTTTTTTTTTTTTT177750.17078079441941207No Hit
TATTTTTTTTTTTTTTTTTTTTTAT175180.16831155874201187No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC173700.1668895864452989No Hit
TTTTTTTTTATTTTTTTTTTTTTTT168300.1617013091464813No Hit
TATTTATTTTTTTTTTTTTTTTTTT166290.15977011704081032No Hit
TTTTTATTTTTTTTTTTTTTTTTAA164560.15810794672100395No Hit
TTTTTATTTTTTTTTTTTTTTTTAT163620.15720480215417273No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA160650.15435124963982305No Hit
TTTATATTTTTTTTTTTTTTTTTTT158700.1524777050596945No Hit
AATTTTTTTTTTTTTTTTTTTTTTT153420.14740472281196174No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC151550.14560804159922308No Hit
TTTTTTTTATTTTTTTTTTTTTTTA150320.1444262673256035No Hit
TTTATTTTTTTTTTTTTTTTTTTAA143960.13831562961810726No Hit
TTTATTTTTTTTTTTTTTTTTTTAT140610.1350969761086556No Hit
TTTTTTTTTTATTTTTTTTTTTTTT132440.12724730471396306No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT131060.12592141162648748No Hit
TTATTATTTTTTTTTTTTTTTTTTT126800.12182843731297582No Hit
TTATTTTTTTTTTTTTTTTTTTTAA120760.11602525307503912No Hit
TATTATTTTTTTTTTTTTTTTTTTT118210.11357523323948637No Hit
TTTTTTTTTTTATTTTTTTTTTTTT117460.11285463917020615No Hit
TTATTTTTTTTTTTTTTTTTTTTAT112360.10795459949910066No Hit
TAATTTTTTTTTTTTTTTTTTTTTT109400.10511065490567471No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA109210.10492810440812374No Hit
TTTTTATATTTTTTTTTTTTTTTTT104300.10021061523456923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)