FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_NGFR_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_NGFR_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8915362
Sequences flagged as poor quality0
Sequence length25
%GC7

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT6955937.802184588803013No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3161413.546025388537224No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1316861.4770684577922915No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1279301.4349389290081547No Hit
TATTTTTTTTTTTTTTTTTTTTTTT783750.8791005906434309No Hit
TTTTTATTTTTTTTTTTTTTTTTTT696380.7811012048641435No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG573260.6430024938976118No Hit
TTTATTTTTTTTTTTTTTTTTTTTT571680.6412302719732524No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA508110.5699263810039346No Hit
TTATTTTTTTTTTTTTTTTTTTTTT456850.5124301178123782No Hit
TTTTATTTTTTTTTTTTTTTTTTTT415180.46569056870601555No Hit
TTTTTTATTTTTTTTTTTTTTTTTT388690.43597780998685187No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT384300.4310537250198029No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG362820.4069604801240825No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT342240.38387672872957934No Hit
TATTTTTTTTTTTTTTTTTTTTTTA338660.37986118791362594No Hit
TTTTTTTATTTTTTTTTTTTTTTTT320270.35923387070541835No Hit
TTTTTTTTTTTTTTTTTTTTTTATT303940.34091717195555266No Hit
TTTTTATTTTTTTTTTTTTTTTTTA288430.3235202339512406No Hit
TTTTTTTTATTTTTTTTTTTTTTTT275770.30932002536745No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC252010.28266939693531234No Hit
TTTATTTTTTTTTTTTTTTTTTTTA235610.26427418202424086No Hit
TTATTTTTTTTTTTTTTTTTTTTTA199730.224029041109043No Hit
TTTTTTTTTTTTTTTTTTTTTAATT187330.21012046398115972No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC180620.20259412909986158No Hit
TTTTATTTTTTTTTTTTTTTTTTTA176180.1976139611605227No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA170550.19129901848068537No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG170000.190682105785497No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA166660.18693576323653488No Hit
TTTTTTATTTTTTTTTTTTTTTTTA156850.17593228407326592No Hit
TATTTTTTTTTTTTTTTTTTTTTAA147710.16568031673868094No Hit
TTTTTTTTTATTTTTTTTTTTTTTT139640.15662852501109883No Hit
TATTTATTTTTTTTTTTTTTTTTTT137480.1542057406081772No Hit
TATTTTTTTTTTTTTTTTTTTTTAT136110.15266906716743528No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC135980.15252325143948164No Hit
TATATTTTTTTTTTTTTTTTTTTTT134700.15108752734886144No Hit
TTTTTTTATTTTTTTTTTTTTTTTA132980.1491582731020905No Hit
TTTTTATTTTTTTTTTTTTTTTTAT131570.1475767332835167No Hit
TTTATATTTTTTTTTTTTTTTTTTT127460.14296671296129088No Hit
AATTTTTTTTTTTTTTTTTTTTTTT124690.13985971629643304No Hit
TTTTTATTTTTTTTTTTTTTTTTAA118430.13283812816574359No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT115160.12917030177798725No Hit
TTTTTTTTATTTTTTTTTTTTTTTA111780.12537909285119325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA108670.12189073197476445No Hit
TTTTTTTTTTATTTTTTTTTTTTTT104810.11756112651398788No Hit
TTTATTTTTTTTTTTTTTTTTTTAT104070.11673109852409808No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC102710.1152056416778141No Hit
TTATTATTTTTTTTTTTTTTTTTTT102300.1147457613050373No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC100380.11259217516910698No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA99100.11115645107848678No Hit
TTTTTTTTTTTATTTTTTTTTTTTT95420.10702874431795366No Hit
TTTATTTTTTTTTTTTTTTTTTTAA93710.10511070666564074No Hit
TATTATTTTTTTTTTTTTTTTTTTT93590.10497610753214509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)