FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_NGFR_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_NGFR_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10434619
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8899418.528734973457103No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4511574.323655707985122No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2035231.9504593315769363No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1667481.5980267223939848No Hit
TATTTTTTTTTTTTTTTTTTTTTTT918570.8803100525280319No Hit
TTTTTATTTTTTTTTTTTTTTTTTT829160.794624125710771No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA828180.7936849443185228No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG798580.765317832879188No Hit
TTTATTTTTTTTTTTTTTTTTTTTT673660.6456009558183198No Hit
TTATTTTTTTTTTTTTTTTTTTTTT555960.5328033539125866No Hit
TTTTATTTTTTTTTTTTTTTTTTTT492700.4721782366946028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG476590.456739244624073No Hit
TTTTTTATTTTTTTTTTTTTTTTTT460810.44161650751215736No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT454500.43556932936411No Hit
TATTTTTTTTTTTTTTTTTTTTTTA449810.43107467555835055No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT446310.4277204563003211No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC395930.3794388659518857No Hit
TTTTTTTTTTTTTTTTTTTTTTATT386830.3707178958810092No Hit
TTTTTATTTTTTTTTTTTTTTTTTA383950.36795785260583064No Hit
TTTTTTTATTTTTTTTTTTTTTTTT379890.36406695826651647No Hit
TTTTTTTTATTTTTTTTTTTTTTTT327360.31372491894529164No Hit
TTTATTTTTTTTTTTTTTTTTTTTA314010.3009309683468078No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA297960.28554947717784424No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC281790.2700529842057482No Hit
TTATTTTTTTTTTTTTTTTTTTTTA264430.2534160566859221No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG239940.2299461053633103No Hit
TTTTTTTTTTTTTTTTTTTTTAATT239460.22948609815078058No Hit
TTTTATTTTTTTTTTTTTTTTTTTA229410.21985469713843886No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA220730.21153623337852584No Hit
TATTTTTTTTTTTTTTTTTTTTTAA210620.20184733146461792No Hit
TTTTTTATTTTTTTTTTTTTTTTTA207870.19921187347616623No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC203120.19465971876884053No Hit
TTTTTTTATTTTTTTTTTTTTTTTA177560.17016433470163117No Hit
TTTTTATTTTTTTTTTTTTTTTTAA171400.16426090880749936No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC169320.1622675442198704No Hit
TTTTTTTTTATTTTTTTTTTTTTTT165910.1589995763141903No Hit
TATTTTTTTTTTTTTTTTTTTTTAT164450.1576003877094123No Hit
TTTTTATTTTTTTTTTTTTTTTTAT160370.1536903264029094No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC159700.15304823300208661No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA153550.14715439059154917No Hit
TATATTTTTTTTTTTTTTTTTTTTT150480.1442122611280776No Hit
TTTTTTTTATTTTTTTTTTTTTTTA150460.14419309416088885No Hit
TATTTATTTTTTTTTTTTTTTTTTT148460.1422763974420149No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT139730.13391001626413002No Hit
TTTATATTTTTTTTTTTTTTTTTTT139070.13327750634690158No Hit
TTTATTTTTTTTTTTTTTTTTTTAA136350.130670798809233No Hit
AATTTTTTTTTTTTTTTTTTTTTTT134000.12841868016455607No Hit
TTTTTTTTTTATTTTTTTTTTTTTT129700.12429778221897704No Hit
TTTATTTTTTTTTTTTTTTTTTTAT128260.12291776058138779No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA123490.11834643890687335No Hit
TTATTTTTTTTTTTTTTTTTTTTAA122830.11771392898964494No Hit
TTATTATTTTTTTTTTTTTTTTTTT114390.10962546883599679No Hit
TTTTTTTTTTTATTTTTTTTTTTTT112830.1081304453952751No Hit
TTATTTTTTTTTTTTTTTTTTTTAT106570.10213118466519958No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA106150.10172867835423605No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA105920.10150825823156552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)