FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_NGFR_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_NGFR_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9061211
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT7355228.117259381775792No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3926934.3337805509660905No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1769691.9530391688263302No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1351461.4914783465477186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG771660.8516080245786132No Hit
TATTTTTTTTTTTTTTTTTTTTTTT753000.8310147506773653No Hit
TTTTTATTTTTTTTTTTTTTTTTTT687550.7587837872884761No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA686830.757989191510936No Hit
TTTATTTTTTTTTTTTTTTTTTTTT557760.6155468623344054No Hit
TTATTTTTTTTTTTTTTTTTTTTTT452640.4995358788135493No Hit
TTTTATTTTTTTTTTTTTTTTTTTT425420.4694957440015468No Hit
TTTTTTATTTTTTTTTTTTTTTTTT401440.4430312901884748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG401280.44285471334902143No Hit
TATTTTTTTTTTTTTTTTTTTTTTA390060.43047226248235476No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT375970.4149224645579934No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT368930.40715308362204566No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC361490.3989422605874645No Hit
TTTTTATTTTTTTTTTTTTTTTTTA344920.3806555216515762No Hit
TTTTTTTATTTTTTTTTTTTTTTTT328900.36297576560130873No Hit
TTTTTTTTTTTTTTTTTTTTTTATT302340.3336640102520513No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC280820.3099144253455747No Hit
TTTATTTTTTTTTTTTTTTTTTTTA280680.30975992061105295No Hit
TTTTTTTTATTTTTTTTTTTTTTTT280230.3092632982500904No Hit
TTATTTTTTTTTTTTTTTTTTTTTA238100.26276840921152816No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA237740.2623711113227581No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG229350.2531118633039226No Hit
TTTTATTTTTTTTTTTTTTTTTTTA215670.2380145435306605No Hit
TTTTTTATTTTTTTTTTTTTTTTTA197850.21834829803654277No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA195790.21607487122858082No Hit
TTTTTTTTTTTTTTTTTTTTTAATT186940.20630796479631694No Hit
TATTTTTTTTTTTTTTTTTTTTTAA186430.20574512612055937No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC185690.20492845823808759No Hit
TTTTTTTATTTTTTTTTTTTTTTTA168170.18559329431794494No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA167220.1845448693336906No Hit
TTTTTATTTTTTTTTTTTTTTTTAA154850.17089327243345287No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC150840.1664678153946531No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC146040.16117051021105236No Hit
TTTTTTTTATTTTTTTTTTTTTTTA139380.15382049926880637No Hit
TTTTTTTTTATTTTTTTTTTTTTTT139030.15343423743250212No Hit
TTTTTATTTTTTTTTTTTTTTTTAT133200.1470002188449204No Hit
TATTTTTTTTTTTTTTTTTTTTTAT132950.14672431753327453No Hit
TTTATTTTTTTTTTTTTTTTTTTAA124860.13779615108841411No Hit
TATTTATTTTTTTTTTTTTTTTTTT123160.1359200221692222No Hit
TATATTTTTTTTTTTTTTTTTTTTT122760.1354785800705888No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT122430.13511439033921624No Hit
TTTATATTTTTTTTTTTTTTTTTTT117220.1293646070045163No Hit
AATTTTTTTTTTTTTTTTTTTTTTT112010.12361482366981631No Hit
TTATTTTTTTTTTTTTTTTTTTTAA107470.11860445585032729No Hit
TTTTTTTTTTATTTTTTTTTTTTTT105910.11688283166565705No Hit
TTTATTTTTTTTTTTTTTTTTTTAT104620.11545918089756436No Hit
TTTTATTTTTTTTTTTTTTTTTTAA96870.10690624023654233No Hit
TTTTTTTTTTTATTTTTTTTTTTTT95320.10519565210433794No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA93940.10367267686405272No Hit
TTATTATTTTTTTTTTTTTTTTTTT93600.10329745108021433No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA92810.10242560293541338No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA92300.1018627642596558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)