FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_NGFR_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_NGFR_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14004180
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT13541809.669827151607592No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA6487944.63285961762845No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2855532.039055482006087No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2280761.628628023918573No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1452501.0371903246030827No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1248010.8911696364942467No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1205670.8609358063092591No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1086650.7759468958553803No Hit
TTTATTTTTTTTTTTTTTTTTTTTT1000990.7147794444230223No Hit
TTATTTTTTTTTTTTTTTTTTTTTT807490.5766064132280505No Hit
TTTTATTTTTTTTTTTTTTTTTTTT741490.5294776273941066No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT715920.5112187932460165No Hit
TATTTTTTTTTTTTTTTTTTTTTTA682290.4872045346460842No Hit
TTTTTTATTTTTTTTTTTTTTTTTT677870.4840483341402353No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC639740.4568207492334431No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT581930.4155402172779842No Hit
TTTTTTTATTTTTTTTTTTTTTTTT574120.4099633109543008No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG553070.3949320845633233No Hit
TTTTTATTTTTTTTTTTTTTTTTTA534160.3814289733493857No Hit
TTTTTTTTTTTTTTTTTTTTTTATT512970.36629777680663916No Hit
TTTTTTTTATTTTTTTTTTTTTTTT488830.34906006635161785No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC475560.3395843241089446No Hit
TTTATTTTTTTTTTTTTTTTTTTTA442410.3159128203150774No Hit
TTATTTTTTTTTTTTTTTTTTTTTA363630.2596581877696516No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG362650.25895839670726883No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA359890.2569875565723948No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA343020.2449411532842337No Hit
TTTTATTTTTTTTTTTTTTTTTTTA326930.23345172655592827No Hit
TATTTTTTTTTTTTTTTTTTTTTAA314970.22491141930480757No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC305620.2182348413116655No Hit
TTTTTTTTTTTTTTTTTTTTTAATT297660.2125508241110868No Hit
TTTTTTATTTTTTTTTTTTTTTTTA295620.21109411618531038No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA267100.19072876812494555No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC266630.19039315404400686No Hit
TTTTTTTATTTTTTTTTTTTTTTTA256880.18343094704581062No Hit
TTTTTATTTTTTTTTTTTTTTTTAA235670.1682854690528114No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC232680.1661503922400312No Hit
TATTTTTTTTTTTTTTTTTTTTTAT231350.16520067579822595No Hit
TATATTTTTTTTTTTTTTTTTTTTT231180.16507928347107792No Hit
TATTTATTTTTTTTTTTTTTTTTTT230040.16426524080667343No Hit
TTTTTTTTTATTTTTTTTTTTTTTT229340.16376539004782856No Hit
AATTTTTTTTTTTTTTTTTTTTTTT224050.1599879464559867No Hit
TTTTTTTTATTTTTTTTTTTTTTTA214820.15339705716436094No Hit
TTTTTATTTTTTTTTTTTTTTTTAT211820.1512548396264544No Hit
TTTATATTTTTTTTTTTTTTTTTTT202020.14425692900262635No Hit
TTTATTTTTTTTTTTTTTTTTTTAA191280.13658779021692094No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT173960.12422005429807387No Hit
TTTATTTTTTTTTTTTTTTTTTTAT173370.12379875151561891No Hit
TTTTTTTTTTATTTTTTTTTTTTTT172780.12337744873316396No Hit
TTATTATTTTTTTTTTTTTTTTTTT163440.11670801146514825No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA163170.11651521188673668No Hit
TTATTTTTTTTTTTTTTTTTTTTAA162360.11593681315150192No Hit
TATTATTTTTTTTTTTTTTTTTTTT161080.11502280033532845No Hit
TTTTTTTTTTTATTTTTTTTTTTTT158040.1128520198969165No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA150490.10746077242651836No Hit
TTTTTTTTTTTTTTTTTTTTTTACC148880.1063111156811752No Hit
TAATTTTTTTTTTTTTTTTTTTTTT146800.10482584485489332No Hit
TTTTATTTTTTTTTTTTTTTTTTAA142720.10191242900334044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)