FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_LTBR_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_LTBR_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9561831
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8162268.53629393784517No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4038194.223239251980086No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1766741.8477005084068105No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1594221.6672748137882796No Hit
TATTTTTTTTTTTTTTTTTTTTTTT900670.9419430232556922No Hit
TTTTTATTTTTTTTTTTTTTTTTTT777280.8128987010960558No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG735100.7687858110020979No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA697140.7290863015671372No Hit
TTTATTTTTTTTTTTTTTTTTTTTT620540.6489761218327327No Hit
TTATTTTTTTTTTTTTTTTTTTTTT489070.5114815352833573No Hit
TTTTATTTTTTTTTTTTTTTTTTTT460780.4818951516712647No Hit
TTTTTTATTTTTTTTTTTTTTTTTT434350.454254002188493No Hit
TATTTTTTTTTTTTTTTTTTTTTTA430400.4501229942256876No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT429150.4488157132247997No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT425410.4449043284701434No Hit
TTTTTTTTTTTTTTTTTTTTTTATT376110.39334516579512857No Hit
TTTTTTTATTTTTTTTTTTTTTTTT361070.3776159607924465No Hit
TTTTTATTTTTTTTTTTTTTTTTTA348990.36498239719986686No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC340160.3557477642095954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG333060.34832240812455273No Hit
TTTTTTTTATTTTTTTTTTTTTTTT300800.31458410005364035No Hit
TTTATTTTTTTTTTTTTTTTTTTTA279180.29197336786228495No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA244210.25540087458144783No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC240830.25186598675504723No Hit
TTTTTTTTTTTTTTTTTTTTTAATT230270.2408220768595471No Hit
TTATTTTTTTTTTTTTTTTTTTTTA228910.23939975513058115No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG225860.23620998948841493No Hit
TTTTATTTTTTTTTTTTTTTTTTTA210660.22031345251761927No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA208380.21792897197199992No Hit
TATTTTTTTTTTTTTTTTTTTTTAA196420.20542090735550544No Hit
TTTTTTATTTTTTTTTTTTTTTTTA189790.19848708892679653No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC182360.19071661065751946No Hit
TATTTTTTTTTTTTTTTTTTTTTAT166470.17409845457423376No Hit
TTTTTTTATTTTTTTTTTTTTTTTA164780.17233101066103343No Hit
TTTTTATTTTTTTTTTTTTTTTTAT154950.16205055287005177No Hit
TTTTTATTTTTTTTTTTTTTTTTAA151670.1586202475237222No Hit
TATTTATTTTTTTTTTTTTTTTTTT150000.15687372010653608No Hit
TTTTTTTTTATTTTTTTTTTTTTTT149600.15645539018625199No Hit
TATATTTTTTTTTTTTTTTTTTTTT147420.15417549212070367No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC142940.1494901970135218No Hit
AATTTTTTTTTTTTTTTTTTTTTTT140640.14708479997188822No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA138500.1448467348983683No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC136550.14280737653698333No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT135460.1416674275042092No Hit
TTTTTTTTATTTTTTTTTTTTTTTA134080.14022418927922906No Hit
TTTATATTTTTTTTTTTTTTTTTTT133170.13927248871058273No Hit
TTTATTTTTTTTTTTTTTTTTTTAT120880.12641930190985387No Hit
TTTATTTTTTTTTTTTTTTTTTTAA118300.12372107392402144No Hit
TTTTTTTTTTATTTTTTTTTTTTTT113250.11843965868043474No Hit
TTATTATTTTTTTTTTTTTTTTTTT103830.10858798905774428No Hit
TATTATTTTTTTTTTTTTTTTTTTT102470.10716566732877834No Hit
TTTTTTTTTTTATTTTTTTTTTTTT102320.10700879360867181No Hit
TTATTTTTTTTTTTTTTTTTTTTAA100020.10460339656703826No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA98640.10316015834205812No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA98440.10295099338191607No Hit
TTATTTTTTTTTTTTTTTTTTTTAT95820.10021093240405526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)