FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_LTBR_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_LTBR_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12076611
Sequences flagged as poor quality0
Sequence length25
%GC8

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT11195889.270713447671703No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5013104.151081789419234No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2146161.7771210814027212No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1980431.639888872797178No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1178170.975579986802589No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1040070.8612267133552617No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG932820.7724186860038798No Hit
TTTATTTTTTTTTTTTTTTTTTTTT851420.7050156703730873No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA826970.6847699242775974No Hit
TTATTTTTTTTTTTTTTTTTTTTTT674800.5587660312980189No Hit
TTTTATTTTTTTTTTTTTTTTTTTT638360.5285920031704259No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG605640.5014983094180975No Hit
TTTTTTATTTTTTTTTTTTTTTTTT596080.49358218129241727No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT563990.46701015707138366No Hit
TATTTTTTTTTTTTTTTTTTTTTTA515280.42667599378666743No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT510060.422353589098796No Hit
TTTTTTTATTTTTTTTTTTTTTTTT499630.41371706019180376No Hit
TTTTTTTTTTTTTTTTTTTTTTATT476710.3947382258151728No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC456960.37838430003251744No Hit
TTTTTTTTATTTTTTTTTTTTTTTT431040.3569213250306729No Hit
TTTTTATTTTTTTTTTTTTTTTTTA429430.35558816956180833No Hit
TTTATTTTTTTTTTTTTTTTTTTTA346950.28729086330593906No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC316490.26206855549127156No Hit
TTATTTTTTTTTTTTTTTTTTTTTA283290.234577399238909No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG280930.23262320861374108No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA274780.22753072033205343No Hit
TTTTTTTTTTTTTTTTTTTTTAATT266900.22100571095649266No Hit
TTTTATTTTTTTTTTTTTTTTTTTA260160.21542467501851303No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA250490.20741746173657494No Hit
TTTTTTATTTTTTTTTTTTTTTTTA234750.19438400392295488No Hit
TATTTTTTTTTTTTTTTTTTTTTAA227710.1885545539224539No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC225510.18673285079729735No Hit
TTTTTTTATTTTTTTTTTTTTTTTA207470.17179488517101363No Hit
TTTTTTTTTATTTTTTTTTTTTTTT202990.16808523517069485No Hit
TATTTTTTTTTTTTTTTTTTTTTAT199370.16508770548293722No Hit
TTTTTATTTTTTTTTTTTTTTTTAT194760.16127040938885917No Hit
TATTTATTTTTTTTTTTTTTTTTTT193350.16010286329500883No Hit
TATATTTTTTTTTTTTTTTTTTTTT192830.15967227891997185No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC189540.15694800470098771No Hit
TTTTTATTTTTTTTTTTTTTTTTAA178800.14805478126272345No Hit
TTTATATTTTTTTTTTTTTTTTTTT178350.14768216016894142No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA175550.1453636289187422No Hit
TTTTTTTTATTTTTTTTTTTTTTTA173100.14333491407481785No Hit
AATTTTTTTTTTTTTTTTTTTTTTT171660.14214252657471538No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC167390.13860676641816153No Hit
TTTATTTTTTTTTTTTTTTTTTTAT155150.12847147266729053No Hit
TTTTTTTTTTATTTTTTTTTTTTTT152290.12610325860458702No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT144260.11945404219776558No Hit
TTTATTTTTTTTTTTTTTTTTTTAA142910.11833617891641951No Hit
TTATTATTTTTTTTTTTTTTTTTTT139680.11566158750993967No Hit
TTTTTTTTTTTATTTTTTTTTTTTT136860.11332649532223901No Hit
TATTATTTTTTTTTTTTTTTTTTTT134980.11176976719710521No Hit
TTTTTATATTTTTTTTTTTTTTTTT125290.10374599297766567No Hit
TTATTTTTTTTTTTTTTTTTTTTAT124020.1026943734463253No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC122540.10146886407121998No Hit
TAATTTTTTTTTTTTTTTTTTTTTT122020.10103827969618298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)