FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_LTBR_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_LTBR_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11375031
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT9664908.496592229067332No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4596054.04047250508592No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1964051.7266326570890227No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1935701.701709648087992No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1086580.955232561564008No Hit
TTTTTATTTTTTTTTTTTTTTTTTT921220.8098615291685799No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG784690.6898354826461571No Hit
TTTATTTTTTTTTTTTTTTTTTTTT770490.6773520001835599No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA764810.672358607198521No Hit
TTATTTTTTTTTTTTTTTTTTTTTT577660.507831583052389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG575590.5060118077919964No Hit
TTTTATTTTTTTTTTTTTTTTTTTT547870.4816426434354332No Hit
TATTTTTTTTTTTTTTTTTTTTTTA500600.4400867127307169No Hit
TTTTTTATTTTTTTTTTTTTTTTTT495070.4352251875181703No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT493610.43394167453257926No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT487960.42897465510203886No Hit
TTTTTTTTTTTTTTTTTTTTTTATT463320.4073131756739828No Hit
TTTTTTTATTTTTTTTTTTTTTTTT416570.3662143865805728No Hit
TTTTTATTTTTTTTTTTTTTTTTTA398130.35000344174886205No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC396450.3485265226969491No Hit
TTTTTTTTATTTTTTTTTTTTTTTT365480.32130022326972124No Hit
TTTATTTTTTTTTTTTTTTTTTTTA331850.2917354686769645No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC266050.23388947247704206No Hit
TTTTTTTTTTTTTTTTTTTTTAATT260870.22933563873364388No Hit
TTATTTTTTTTTTTTTTTTTTTTTA260250.22879058527400936No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA251560.22115104565429317No Hit
TTTTATTTTTTTTTTTTTTTTTTTA241680.21246535503947195No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG237960.20919503428166483No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA228520.20089615579948747No Hit
TATTTTTTTTTTTTTTTTTTTTTAA220750.19406540518439028No Hit
TATTTTTTTTTTTTTTTTTTTTTAT209670.18432477238963127No Hit
TTTTTTATTTTTTTTTTTTTTTTTA208690.18346323627601543No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC199720.1755775434809804No Hit
TTTTTATTTTTTTTTTTTTTTTTAT193380.17000393229697572No Hit
TATATTTTTTTTTTTTTTTTTTTTT186540.1639907618713303No Hit
TTTTTTTATTTTTTTTTTTTTTTTA184640.16232043675309546No Hit
TATTTATTTTTTTTTTTTTTTTTTT182040.16003472869656354No Hit
TTTTTTTTTATTTTTTTTTTTTTTT171250.1505490402619562No Hit
TTTATATTTTTTTTTTTTTTTTTTT166190.14610070073655185No Hit
TTTTTATTTTTTTTTTTTTTTTTAA163470.14370949846202616No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC162290.14267213865175402No Hit
TTTATTTTTTTTTTTTTTTTTTTAT160490.1410897253818473No Hit
AATTTTTTTTTTTTTTTTTTTTTTT158830.13963038869960004No Hit
TTTTTTTTATTTTTTTTTTTTTTTA156110.13723918642507435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA152920.1344347984634064No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC148010.13011832671049423No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA139910.12299746699591411No Hit
TTTATTTTTTTTTTTTTTTTTTTAA133370.11724803211525313No Hit
TTTTTTTTTTATTTTTTTTTTTTTT129810.114118370314771No Hit
TATTATTTTTTTTTTTTTTTTTTTT126790.11146343249526089No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT126570.11127002642893895No Hit
TTATTATTTTTTTTTTTTTTTTTTT124220.10920409799322744No Hit
AAAAAAAAAAAAAAAAAAAAATTTT123480.10855355031559914No Hit
TTATTTTTTTTTTTTTTTTTTTTAT119520.10507224112180442No Hit
TTTTTTTTTTTATTTTTTTTTTTTT115940.10192499695165665No Hit
TTTTATTTTTTTTTTTTTTTTTTAT114640.10078214292339072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)