FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_LTBR_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_LTBR_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11575011
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT10474569.049287296573628No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5283044.564177088039052No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2393472.0677906915164055No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1761231.521579547526996No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1182331.021450433178854No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1020840.8819343670602128No Hit
TTTTTATTTTTTTTTTTTTTTTTTT952190.8226255681312096No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA939880.8119905890370211No Hit
TTTATTTTTTTTTTTTTTTTTTTTT800390.6914809843377254No Hit
TTATTTTTTTTTTTTTTTTTTTTTT618100.5339951728771575No Hit
TATTTTTTTTTTTTTTTTTTTTTTA579090.5002932610603998No Hit
TTTTATTTTTTTTTTTTTTTTTTTT571990.49415935760233837No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT550490.4755848612152507No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC538230.4649930786242882No Hit
TTTTTTATTTTTTTTTTTTTTTTTT510720.44122636254946107No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT466570.40308385020109266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG450580.38926960846948655No Hit
TTTTTATTTTTTTTTTTTTTTTTTA441330.3812782553727163No Hit
TTTTTTTATTTTTTTTTTTTTTTTT430260.3717145495585274No Hit
TTTTTTTTTTTTTTTTTTTTTTATT395640.3418052907249937No Hit
TTTATTTTTTTTTTTTTTTTTTTTA373790.3229284188153255No Hit
TTTTTTTTATTTTTTTTTTTTTTTT370870.3204057430269397No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC359910.31093706951984756No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA313350.27071248571599626No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG308430.266461949798579No Hit
TTATTTTTTTTTTTTTTTTTTTTTA294190.25415958567987534No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA277130.23942093877923745No Hit
TTTTATTTTTTTTTTTTTTTTTTTA270770.23392634356891756No Hit
TATTTTTTTTTTTTTTTTTTTTTAA268070.23159373239472514No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC256940.22197819077666536No Hit
TTTTTTTTTTTTTTTTTTTTTAATT236880.2046477536824803No Hit
TTTTTTATTTTTTTTTTTTTTTTTA231540.20003436713796643No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC217550.18794798553539172No Hit
TTTTTTTATTTTTTTTTTTTTTTTA201380.17397823639217277No Hit
TTTTTATTTTTTTTTTTTTTTTTAA200440.173166142131528No Hit
TATATTTTTTTTTTTTTTTTTTTTT196590.16984001138314253No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA194460.16799984034572407No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC193110.16683353475862786No Hit
TATTTTTTTTTTTTTTTTTTTTTAT190650.16470826679991923No Hit
TATTTATTTTTTTTTTTTTTTTTTT188500.16285081716121047No Hit
AATTTTTTTTTTTTTTTTTTTTTTT180240.15571475482831076No Hit
TTTTTTTTTATTTTTTTTTTTTTTT175430.15155925121799022No Hit
TTTTTTTTATTTTTTTTTTTTTTTA171650.14829359557412083No Hit
TTTTTATTTTTTTTTTTTTTTTTAT169510.14644478523605722No Hit
TTTATATTTTTTTTTTTTTTTTTTT166180.14356789812121992No Hit
TTTATTTTTTTTTTTTTTTTTTTAA163770.14148582666573709No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT149550.12920074114832375No Hit
TTTATTTTTTTTTTTTTTTTTTTAT139360.12039729379090872No Hit
TTATTTTTTTTTTTTTTTTTTTTAA136300.11775366779349064No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA135800.1173217027612328No Hit
TTTTTTTTTTATTTTTTTTTTTTTT132560.1145225693522019No Hit
TATTATTTTTTTTTTTTTTTTTTTT131380.11350313187607339No Hit
TTATTATTTTTTTTTTTTTTTTTTT129110.11154201062962273No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA127540.11018564042833308No Hit
TTTTTTTTTTTTTTTTTTTTTTACC122360.10571048269414171No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG120800.10436275179349722No Hit
TTTTTTTTTTTATTTTTTTTTTTTT120520.10412085137543282No Hit
TTTTATTTTTTTTTTTTTTTTTTAA120200.1038443937547878No Hit
TAATTTTTTTTTTTTTTTTTTTTTT118100.10203014061930481No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA116270.10044914860124109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)