FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_LTBR_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_LTBR_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6726629
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT67749610.071850253670895No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3332534.954234877529294No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1507962.2417766759546276No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1141751.6973583647916364No Hit
TATTTTTTTTTTTTTTTTTTTTTTT685281.0187569434853625No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG636270.9458972688994741No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA583360.8672397422245228No Hit
TTTTTATTTTTTTTTTTTTTTTTTT576410.8569076724760649No Hit
TTTATTTTTTTTTTTTTTTTTTTTT481330.7155590118021968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG389560.5791310922603283No Hit
TTATTTTTTTTTTTTTTTTTTTTTT382690.5689179528111331No Hit
TTTTATTTTTTTTTTTTTTTTTTTT352050.523367648193471No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT344970.5128423167087109No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC332100.49370940481480396No Hit
TATTTTTTTTTTTTTTTTTTTTTTA331760.49320395104293696No Hit
TTTTTTATTTTTTTTTTTTTTTTTT311640.46329298077833636No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT298810.4442195340340608No Hit
TTTTTATTTTTTTTTTTTTTTTTTA265580.3948188609777647No Hit
TTTTTTTTTTTTTTTTTTTTTTATT260790.387697909309403No Hit
TTTTTTTATTTTTTTTTTTTTTTTT256630.38151353374773606No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC240230.35713282239885685No Hit
TTTTTTTTATTTTTTTTTTTTTTTT221750.3296599232691442No Hit
TTTATTTTTTTTTTTTTTTTTTTTA217240.3229552276482024No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA194920.2897736741538741No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG192450.28610170116413436No Hit
TTATTTTTTTTTTTTTTTTTTTTTA179890.2674296441798708No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA170100.2528755488075825No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC158540.23569012056410427No Hit
TTTTATTTTTTTTTTTTTTTTTTTA157680.23441161984702885No Hit
TTTTTTTTTTTTTTTTTTTTTAATT153090.22758799392682427No Hit
TATTTTTTTTTTTTTTTTTTTTTAA152750.22708254015495724No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC140110.20829155287143084No Hit
TTTTTTATTTTTTTTTTTTTTTTTA137930.20505070221651886No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA130810.1944659055821274No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC123910.1842081672707087No Hit
TTTTTATTTTTTTTTTTTTTTTTAA118690.17644796524380935No Hit
TTTTTTTATTTTTTTTTTTTTTTTA118210.1757343834482324No Hit
TATTTTTTTTTTTTTTTTTTTTTAT110310.1639900163960284No Hit
TATATTTTTTTTTTTTTTTTTTTTT109600.1629345099900708No Hit
TTTTTTTTTATTTTTTTTTTTTTTT106750.15869761807883265No Hit
TATTTATTTTTTTTTTTTTTTTTTT105660.15707719275137666No Hit
AATTTTTTTTTTTTTTTTTTTTTTT103320.15359848149793903No Hit
TTTTTATTTTTTTTTTTTTTTTTAT99740.14827635060592756No Hit
TTTTTTTTATTTTTTTTTTTTTTTA98500.14643293096735377No Hit
TTTATATTTTTTTTTTTTTTTTTTT97310.14466384276581926No Hit
TTTATTTTTTTTTTTTTTTTTTTAA95760.142359568217602No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT89780.1334695283477058No Hit
TTTATTTTTTTTTTTTTTTTTTTAT83680.12440109302891537No Hit
TTTTTTTTTTATTTTTTTTTTTTTT82290.12233467907922378No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA81960.12184409159476464No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA81530.12120484123622693No Hit
TTATTTTTTTTTTTTTTTTTTTTAA81020.12044666057842643No Hit
TTTTTTTTTTTTTTTTTTTTTTACC79660.11842484549095839No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC78160.11619490237978042No Hit
TTATTATTTTTTTTTTTTTTTTTTT76810.11418795357972024No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA75430.1121364059174365No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG74140.11021865484182344No Hit
TTTTTTTTTTTATTTTTTTTTTTTT73950.10993619538107423No Hit
TATTATTTTTTTTTTTTTTTTTTTT73210.10883609011289311No Hit
TTTTATTTTTTTTTTTTTTTTTTAA71420.10617502466688739No Hit
TAATTTTTTTTTTTTTTTTTTTTTT67410.10021364341633826No Hit
TTATTTTTTTTTTTTTTTTTTTTAT67270.10000551539262831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)