FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_LTBR_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_LTBR_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13754632
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT13073669.504914417194149No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA6460364.696861391856939No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2878272.092582338807756No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2215181.610497467325916No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1459841.0613442802395587No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1225470.8909507720744546No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1183580.8604955770536062No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1112300.8086730346547986No Hit
TTTATTTTTTTTTTTTTTTTTTTTT1005730.7311936807905874No Hit
TTATTTTTTTTTTTTTTTTTTTTTT835930.6077443584095888No Hit
TTTTATTTTTTTTTTTTTTTTTTTT719740.5232709969994108No Hit
TATTTTTTTTTTTTTTTTTTTTTTA702710.5108897133707394No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT690160.5017655143372792No Hit
TTTTTTATTTTTTTTTTTTTTTTTT651520.47367315970358204No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC647850.47100496763562993No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT582020.4231447268091214No Hit
TTTTTTTATTTTTTTTTTTTTTTTT558830.40628495186203456No Hit
TTTTTATTTTTTTTTTTTTTTTTTA536590.3901158533358072No Hit
TTTTTTTTTTTTTTTTTTTTTTATT504120.3665092602986398No Hit
TTTTTTTTATTTTTTTTTTTTTTTT460460.33476722605155845No Hit
TTTATTTTTTTTTTTTTTTTTTTTA460250.334614550211158No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC440610.3203357239946514No Hit
TTATTTTTTTTTTTTTTTTTTTTTA392140.2850966859745866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG377170.2742130796374632No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA376870.2739949712940339No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG363300.264129203892914No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA340510.24756024007039956No Hit
TTTTATTTTTTTTTTTTTTTTTTTA329110.23927212302008513No Hit
TATTTTTTTTTTTTTTTTTTTTTAA326660.237490904882079No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC303380.22056569743196328No Hit
TTTTTTTTTTTTTTTTTTTTTAATT296610.2156437191485748No Hit
TTTTTTATTTTTTTTTTTTTTTTTA291160.2116814175762754No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC265640.19312766782855403No Hit
TTTTTTTATTTTTTTTTTTTTTTTA256700.1866280391943601No Hit
TTTTTATTTTTTTTTTTTTTTTTAA242500.1763042442720387No Hit
TATATTTTTTTTTTTTTTTTTTTTT238630.1734906466418004No Hit
TATTTTTTTTTTTTTTTTTTTTTAT236450.1719057260128806No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA234210.17027718371527498No Hit
TATTTATTTTTTTTTTTTTTTTTTT232630.16912847977321385No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC230070.16726728857595027No Hit
TTTTTTTTTATTTTTTTTTTTTTTT227690.16553696238474427No Hit
AATTTTTTTTTTTTTTTTTTTTTTT223670.1626143105827913No Hit
TTTTTATTTTTTTTTTTTTTTTTAT209620.15239956983218453No Hit
TTTTTTTTATTTTTTTTTTTTTTTA207880.15113454144029442No Hit
TTTATATTTTTTTTTTTTTTTTTTT205880.14968048581743226No Hit
TTTATTTTTTTTTTTTTTTTTTTAA202150.1469686720807943No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT180730.13139573635994042No Hit
TTATTTTTTTTTTTTTTTTTTTTAA178750.12995622129330686No Hit
TTTTTTTTTTATTTTTTTTTTTTTT175640.12769516479975618No Hit
TTTATTTTTTTTTTTTTTTTTTTAT175300.12744797534386962No Hit
TTATTATTTTTTTTTTTTTTTTTTT167760.12196618564567921No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA167060.12145726617767746No Hit
TATTATTTTTTTTTTTTTTTTTTTT160470.11666615290034658No Hit
TAATTTTTTTTTTTTTTTTTTTTTT159390.11588096286400101No Hit
TTTTTTTTTTTATTTTTTTTTTTTT157400.11443417751925315No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA155640.11315460857113445No Hit
TTATTTTTTTTTTTTTTTTTTTTAT146610.1065895474339117No Hit
TTTTTTTTTTTTTTTTTTTTTTACC145910.10608062796590996No Hit
TTTTATTTTTTTTTTTTTTTTTTAA143330.10420489621241774No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG142490.10359419285081563No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA140590.10221284000909657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)