FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IL12B_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IL12B_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13432916
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT141455610.530520700047555No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA6788845.053884056149833No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3006862.238426861301001No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2579401.92020853848859No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1421661.0583405717716095No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1305660.9719855316596933No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1233720.9184305179902861No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA1173640.8737045627323211No Hit
TTTATTTTTTTTTTTTTTTTTTTTT1017910.7577729213820737No Hit
TTATTTTTTTTTTTTTTTTTTTTTT790130.588204377962313No Hit
TTTTATTTTTTTTTTTTTTTTTTTT743920.5538038055177297No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT675810.5030999970520176No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT670790.49936290824717433No Hit
TATTTTTTTTTTTTTTTTTTTTTTA664170.4944347154407874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG664080.4943677158407006No Hit
TTTTTTATTTTTTTTTTTTTTTTTT661220.4922386174379413No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC653020.48613420943002994No Hit
TTTTTTTTTTTTTTTTTTTTTTATT604790.4502298681834979No Hit
TTTTTATTTTTTTTTTTTTTTTTTA545240.4058984661260444No Hit
TTTTTTTATTTTTTTTTTTTTTTTT544340.40522847012517615No Hit
TTTTTTTTATTTTTTTTTTTTTTTT465910.34684204084950726No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC450180.33513199963433105No Hit
TTTATTTTTTTTTTTTTTTTTTTTA445080.33133535562941063No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG407800.30358263239344313No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA392740.2923713659789133No Hit
TTTTTTTTTTTTTTTTTTTTTAATT356050.265057862343515No Hit
TTATTTTTTTTTTTTTTTTTTTTTA355930.2649685295433992No Hit
TTTTATTTTTTTTTTTTTTTTTTTA328760.24474209471718575No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC327500.2438041003159701No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA322630.24017867751127156No Hit
TATTTTTTTTTTTTTTTTTTTTTAA304870.22695742309413683No Hit
TTTTTTATTTTTTTTTTTTTTTTTA280570.2088675310706923No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC272710.20301623266310906No Hit
TATTTTTTTTTTTTTTTTTTTTTAT246860.1837724586381691No Hit
TTTTTTTATTTTTTTTTTTTTTTTA240250.1788517102317918No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC234700.1747200682264372No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA234320.1744371810260706No Hit
TTTTTATTTTTTTTTTTTTTTTTAT233630.17392351742540488No Hit
TTTTTATTTTTTTTTTTTTTTTTAA231830.17258352542366825No Hit
TTTTTTTTTATTTTTTTTTTTTTTT224880.16740966741696292No Hit
TATTTATTTTTTTTTTTTTTTTTTT220700.16429790821293008No Hit
TATATTTTTTTTTTTTTTTTTTTTT219980.16376191141223545No Hit
TTTATATTTTTTTTTTTTTTTTTTT202560.1507937665954287No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT201910.1503098805948016No Hit
TTTTTTTTATTTTTTTTTTTTTTTA200600.14933466419353772No Hit
AATTTTTTTTTTTTTTTTTTTTTTT199650.1486274461926212No Hit
TTTATTTTTTTTTTTTTTTTTTTAA190410.14174882058370647No Hit
TTTATTTTTTTTTTTTTTTTTTTAT186530.13886039337996306No Hit
TTTTTTTTTTATTTTTTTTTTTTTT173940.1294878937678163No Hit
TTTTTTTTTTTATTTTTTTTTTTTT160370.11938584295472406No Hit
TTATTATTTTTTTTTTTTTTTTTTT159850.11899873415422235No Hit
TTATTTTTTTTTTTTTTTTTTTTAA156950.11683985815142445No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG155100.11546264414963958No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA154010.11465120454858796No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA153170.11402587494777754No Hit
TATTATTTTTTTTTTTTTTTTTTTT151930.11310276934658119No Hit
TTTTTTTTTTTTTTTTTTTTTTACC150730.11220944134542343No Hit
TTATTTTTTTTTTTTTTTTTTTTAT149880.11157666734460335No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA148750.11073545014351313No Hit
TTTTATTTTTTTTTTTTTTTTTTAT143330.10670058533828397No Hit
TAATTTTTTTTTTTTTTTTTTTTTT140550.10463104213560184No Hit
TTTTTTTTTTTTTTTTTTTTAATTT139760.10404293453483965No Hit
TTTTATTTTTTTTTTTTTTTTTTAA138910.10341016053401957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)