FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IL12B_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IL12B_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11085609
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT10000879.021489031410002No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4929054.446350218558132No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2134601.925559524966107No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1911811.724587255422774No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1078470.972855889108122No Hit
TTTTTATTTTTTTTTTTTTTTTTTT936180.8445002886174318No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG935080.8435080111521162No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA806710.7277092309497836No Hit
TTTATTTTTTTTTTTTTTTTTTTTT788510.7112915492509252No Hit
TTATTTTTTTTTTTTTTTTTTTTTT644820.5816730501680152No Hit
TTTTATTTTTTTTTTTTTTTTTTTT569150.5134133812585308No Hit
TTTTTTATTTTTTTTTTTTTTTTTT523880.47257665320867803No Hit
TATTTTTTTTTTTTTTTTTTTTTTA510410.46042576461067675No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT496750.4481034826323028No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT496440.44782384080116844No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC476910.4302064054397012No Hit
TTTTTTTATTTTTTTTTTTTTTTTT445650.40200768401627734No Hit
TTTTTTTTTTTTTTTTTTTTTTATT441210.39800249133809423No Hit
TTTTTATTTTTTTTTTTTTTTTTTA420060.378923701891344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG377000.34008054947635263No Hit
TTTTTTTTATTTTTTTTTTTTTTTT368590.3324941372188032No Hit
TTTATTTTTTTTTTTTTTTTTTTTA351550.3171228572106413No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC325580.2936960883249626No Hit
TTATTTTTTTTTTTTTTTTTTTTTA298160.2689613173259133No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG277080.24994567280877394No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA268790.24246750900198624No Hit
TTTTATTTTTTTTTTTTTTTTTTTA262440.23673936181584612No Hit
TTTTTTTTTTTTTTTTTTTTTAATT253120.2283320654733538No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA241330.2176966551860164No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC231180.2085406403924223No Hit
TATTTTTTTTTTTTTTTTTTTTTAA230370.2078099633497808No Hit
TTTTTTATTTTTTTTTTTTTTTTTA229920.2074040316594244No Hit
TTTTTTTATTTTTTTTTTTTTTTTA205010.1849334574221407No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC197280.17796045305224098No Hit
TATTTTTTTTTTTTTTTTTTTTTAT196780.17750941784073387No Hit
TTTTTATTTTTTTTTTTTTTTTTAT184720.16663044853918266No Hit
TTTTTTTTTATTTTTTTTTTTTTTT181520.1637438231855372No Hit
TTTTTATTTTTTTTTTTTTTTTTAA179650.16205695149450067No Hit
TATATTTTTTTTTTTTTTTTTTTTT177250.1598919824792666No Hit
TATTTATTTTTTTTTTTTTTTTTTT173720.15670767388602647No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC172450.15556204444879843No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA168620.15210711472865404No Hit
TTTTTTTTATTTTTTTTTTTTTTTA164340.1482462533181533No Hit
TTTATATTTTTTTTTTTTTTTTTTT163810.14776815599395576No Hit
TTTATTTTTTTTTTTTTTTTTTTAT151290.13647423429781802No Hit
AATTTTTTTTTTTTTTTTTTTTTTT151200.13639304795974674No Hit
TTTATTTTTTTTTTTTTTTTTTTAA148550.1340025613387591No Hit
TTTTTTTTTTATTTTTTTTTTTTTT142400.1284548282372218No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT138020.1245037597844196No Hit
TTATTATTTTTTTTTTTTTTTTTTT134710.12151790668424262No Hit
TTATTTTTTTTTTTTTTTTTTTTAA130370.11760292104836098No Hit
TTATTTTTTTTTTTTTTTTTTTTAT128130.11558228330080919No Hit
TTTTTTTTTTTATTTTTTTTTTTTT125900.11357066625748752No Hit
TATTATTTTTTTTTTTTTTTTTTTT123060.1110087862561272No Hit
TAATTTTTTTTTTTTTTTTTTTTTT117200.10572265357726401No Hit
TTTTATTTTTTTTTTTTTTTTTTAT114660.10343139470280795No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA114490.10327804273089553No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA113100.10202416484290579No Hit
TTTTTATATTTTTTTTTTTTTTTTT112130.10114915653258202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)