FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IL12B_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IL12B_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10186983
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT9384149.211893256325252No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4781264.693499537596166No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2116792.0779361269180483No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1730861.6990899071884187No Hit
TATTTTTTTTTTTTTTTTTTTTTTT983380.9653299706105332No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG947570.9301772664193118No Hit
TTTTTATTTTTTTTTTTTTTTTTTT875470.8594006684805502No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA808990.7941409149303577No Hit
TTTATTTTTTTTTTTTTTTTTTTTT725950.7126251216871571No Hit
TTATTTTTTTTTTTTTTTTTTTTTT569220.5587719150998878No Hit
TTTTATTTTTTTTTTTTTTTTTTTT521350.5117805733061497No Hit
TTTTTTATTTTTTTTTTTTTTTTTT493580.48452029418327286No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC486970.4780316213347956No Hit
TATTTTTTTTTTTTTTTTTTTTTTA484880.4759799834749896No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT464490.4559642437805187No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT451460.4431734106162737No Hit
TTTTTATTTTTTTTTTTTTTTTTTA410580.40304376673643216No Hit
TTTTTTTATTTTTTTTTTTTTTTTT410400.40286707065281246No Hit
TTTTTTTTTTTTTTTTTTTTTTATT392790.3855803038053563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG347050.3406798656677841No Hit
TTTTTTTTATTTTTTTTTTTTTTTT345820.3394724424297164No Hit
TTTATTTTTTTTTTTTTTTTTTTTA341450.33518265417739485No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC322670.31674736278641086No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG280080.2749391061121826No Hit
TTATTTTTTTTTTTTTTTTTTTTTA273830.26880382543094455No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA267170.2622660703370173No Hit
TTTTATTTTTTTTTTTTTTTTTTTA244460.23997291445367092No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC234840.23052949042910936No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA234190.22989142123826062No Hit
TATTTTTTTTTTTTTTTTTTTTTAA224030.21991790896284014No Hit
TTTTTTTTTTTTTTTTTTTTTAATT223480.2193780042628912No Hit
TTTTTTATTTTTTTTTTTTTTTTTA222970.21887736535930213No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC198100.19446385647251987No Hit
TTTTTTTATTTTTTTTTTTTTTTTA195150.19156800399097557No Hit
TTTTTATTTTTTTTTTTTTTTTTAA178510.17523343270524747No Hit
TATTTTTTTTTTTTTTTTTTTTTAT171820.1686662282640503No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC171630.16847971573134066No Hit
TATATTTTTTTTTTTTTTTTTTTTT165740.16269782721734197No Hit
TTTTTATTTTTTTTTTTTTTTTTAT165420.1623837008464626No Hit
TTTTTTTTTATTTTTTTTTTTTTTT162970.1599786708194173No Hit
TTTTTTTTATTTTTTTTTTTTTTTA161190.1582313428814007No Hit
TATTTATTTTTTTTTTTTTTTTTTT161010.15805464679778106No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA160340.15739694470875235No Hit
TTTATATTTTTTTTTTTTTTTTTTT152530.14973029796947732No Hit
TTTATTTTTTTTTTTTTTTTTTTAA147690.14497913660992662No Hit
AATTTTTTTTTTTTTTTTTTTTTTT144140.14149429718298343No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT141720.13911871650320806No Hit
TTTATTTTTTTTTTTTTTTTTTTAT136270.13376875174916852No Hit
TTTTTTTTTTATTTTTTTTTTTTTT129000.12663219326075248No Hit
TTATTTTTTTTTTTTTTTTTTTTAA122530.12028095069953489No Hit
TTATTATTTTTTTTTTTTTTTTTTT119180.1169924402543913No Hit
TTTTTTTTTTTATTTTTTTTTTTTT115800.1136744804619778No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA113150.11107312145313289No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA111600.10955157184418586No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG110640.1086091927315477No Hit
TTTTTTTTTTTTTTTTTTTTTTACC109490.10748030108619991No Hit
TATTATTTTTTTTTTTTTTTTTTTT108990.10698947863170087No Hit
TTATTTTTTTTTTTTTTTTTTTTAT107400.10542866322639391No Hit
TTTTATTTTTTTTTTTTTTTTTTAA106180.10423105643741624No Hit
TTTTTATATTTTTTTTTTTTTTTTT101880.10000998332872452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)