FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IL12B_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IL12B_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10919149
Sequences flagged as poor quality0
Sequence length25
%GC5

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT9807408.981835489194259No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4874054.463763613812762No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2116311.9381638624035629No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1815471.6626478858379898No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1053320.9646539304482428No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG921650.8440676100307817No Hit
TTTTTATTTTTTTTTTTTTTTTTTT914980.8379590753821566No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA785720.7195798866743186No Hit
TTTATTTTTTTTTTTTTTTTTTTTT767810.7031775095293599No Hit
TTATTTTTTTTTTTTTTTTTTTTTT607590.5564444628422965No Hit
TTTTATTTTTTTTTTTTTTTTTTTT546650.5006342527242736No Hit
TTTTTTATTTTTTTTTTTTTTTTTT514050.47077844619576126No Hit
TATTTTTTTTTTTTTTTTTTTTTTA513770.47052201595563903No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT509130.4662726005478998No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT465560.42637022354031434No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC462820.42386087047626153No Hit
TTTTTTTATTTTTTTTTTTTTTTTT425880.39003039522585503No Hit
TTTTTATTTTTTTTTTTTTTTTTTA424030.3883361239964763No Hit
TTTTTTTTTTTTTTTTTTTTTTATT409570.3750933337387373No Hit
TTTTTTTTATTTTTTTTTTTTTTTT366520.33566718431995024No Hit
TTTATTTTTTTTTTTTTTTTTTTTA357750.3276354228704087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG349180.31978682587809726No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC344690.3156747838132807No Hit
TTATTTTTTTTTTTTTTTTTTTTTA292580.2679512844819683No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG261070.23909372424536016No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA259290.23746356057601192No Hit
TTTTATTTTTTTTTTTTTTTTTTTA258800.23701480765579808No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA256830.23521063775208123No Hit
TTTTTTTTTTTTTTTTTTTTTAATT234890.2151174967939351No Hit
TTTTTTATTTTTTTTTTTTTTTTTA234080.21437568074215307No Hit
TATTTTTTTTTTTTTTTTTTTTTAA228550.20931118349973976No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC226600.20752533004174592No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA202890.18581118363711313No Hit
TTTTTTTATTTTTTTTTTTTTTTTA200390.18352162792173637No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC193580.1772848781530502No Hit
TATTTTTTTTTTTTTTTTTTTTTAT187370.17159762175605445No Hit
TTTTTATTTTTTTTTTTTTTTTTAA181450.16617595382204237No Hit
TTTTTATTTTTTTTTTTTTTTTTAT177630.16267751268894673No Hit
TATATTTTTTTTTTTTTTTTTTTTT175860.16105650724246No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC175210.16046122275646207No Hit
TATTTATTTTTTTTTTTTTTTTTTT174650.15994836227621767No Hit
TTTTTTTTATTTTTTTTTTTTTTTA171930.1574573256578878No Hit
TTTTTTTTTATTTTTTTTTTTTTTT171770.15731079409210372No Hit
TTTATATTTTTTTTTTTTTTTTTTT162450.1487753303851793No Hit
AATTTTTTTTTTTTTTTTTTTTTTT161850.14822583701348888No Hit
TTTATTTTTTTTTTTTTTTTTTTAA150070.13743745048263376No Hit
TTTATTTTTTTTTTTTTTTTTTTAT145720.13345362353787826No Hit
TTTTTTTTTTATTTTTTTTTTTTTT131700.12061379508604562No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT128450.1176373726560559No Hit
TTATTATTTTTTTTTTTTTTTTTTT126670.11600720898670767No Hit
TTATTTTTTTTTTTTTTTTTTTTAA124600.11411145685437574No Hit
TTTTTTTTTTTATTTTTTTTTTTTT119290.10924844051491558No Hit
TATTATTTTTTTTTTTTTTTTTTTT118880.1088729533775938No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA118730.1087355800346712No Hit
TTATTTTTTTTTTTTTTTTTTTTAT117980.10804871332005818No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA112880.10337801966068967No Hit
TAATTTTTTTTTTTTTTTTTTTTTT112080.10264536183176912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)