FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IL12B_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IL12B_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13611723
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT163825312.035603428015689No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA6648474.884370626701704No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA3146722.3117719924215323No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG2200831.6168636402606782No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1624701.1936034842907104No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1268910.9322185001854653No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1134180.833237643757517No Hit
TTTATTTTTTTTTTTTTTTTTTTTT1120130.822915658803812No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC1066530.7835378372010655No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA934850.6867976963680498No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA934520.6865552582872866No Hit
TTATTTTTTTTTTTTTTTTTTTTTT931910.6846377934667051No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC880080.6465603215698703No Hit
TTTTATTTTTTTTTTTTTTTTTTTT780830.5736452321282177No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT776740.5706404692484559No Hit
TATTTTTTTTTTTTTTTTTTTTTTA677800.49795312467055053No Hit
TTTTTTATTTTTTTTTTTTTTTTTT676150.49674093426673466No Hit
TTTTTTTATTTTTTTTTTTTTTTTT580090.4261694129391261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG579520.42575065625417147No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG522330.38373540219706204No Hit
TTTTTTTTTTTTTTTTTTTTTTGAC506420.3720469480608737No Hit
TTTTTATTTTTTTTTTTTTTTTTTA501280.3682707912877745No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC488490.3588744790060744No Hit
TTTTTTTTATTTTTTTTTTTTTTTT482100.3541799961694783No Hit
TTTATTTTTTTTTTTTTTTTTTTTA446190.32779832501733985No Hit
TTATTTTTTTTTTTTTTTTTTTTTA373450.27435909473032916No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC357910.2629424651089359No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC349260.25658764874953743No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA326870.24013859230018123No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT320870.2357306271953962No Hit
TTTTATTTTTTTTTTTTTTTTTTTA313740.23049249532920996No Hit
TATTTTTTTTTTTTTTTTTTTTTAA307930.22622411578607649No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA304430.22365280280828517No Hit
TTTTTTATTTTTTTTTTTTTTTTTA266600.19586058282261548No Hit
TATATTTTTTTTTTTTTTTTTTTTT249370.18320237636337441No Hit
TTTTTTTTTTTTTTTTTTTTTTATT239450.17591454072346316No Hit
TTTTTATTTTTTTTTTTTTTTTTAA236660.1738648369497381No Hit
AATTTTTTTTTTTTTTTTTTTTTTT234160.17202818482274432No Hit
TTTTTTTATTTTTTTTTTTTTTTTA234150.17202083821423636No Hit
TTTTTTTTTATTTTTTTTTTTTTTT234040.17194002552064863No Hit
TATTTATTTTTTTTTTTTTTTTTTT231930.17038989112546588No Hit
TTTTTTTTTTTTTTTTTTTTTTACC214710.15773903127473282No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA208350.15306658826366065No Hit
TTTATTTTTTTTTTTTTTTTTTTAA204330.15011325164345468No Hit
TTTATATTTTTTTTTTTTTTTTTTT200620.14738765988699593No Hit
TTTTTTTTATTTTTTTTTTTTTTTA192100.14112834943820118No Hit
TTTTTTTTTTATTTTTTTTTTTTTT186810.13724199353748234No Hit
TTATTTTTTTTTTTTTTTTTTTTAA176560.1297117198168079No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC176430.12961621390620423No Hit
TATTTTTTTTTTTTTTTTTTTTTTG176380.12957948086366436No Hit
TTTTTTTTTTTTTTTTTTTTTTCAC175940.12925623008931345No Hit
TTTTTTTTTTTATTTTTTTTTTTTT168580.1238491262274438No Hit
TTATTATTTTTTTTTTTTTTTTTTT165550.12162310384952735No Hit
TAATTTTTTTTTTTTTTTTTTTTTT163110.11983053137358145No Hit
TTTTTTTTTTTTTTTTTTTTTTGCC161070.1183318232379545No Hit
TATTATTTTTTTTTTTTTTTTTTTT160700.11805999872315945No Hit
TTTTTATTTTTTTTTTTTTTTTTTG154590.11357122092478668No Hit
TTTTTTTTTTTTTTTTTTTTTAATT154260.1133287828440235No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA152950.11236637712947876No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT152670.11216067209125545No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG148870.10936896085822494No Hit
TTTTATTTTTTTTTTTTTTTTTTAA146240.10743680282062748No Hit
TTTTTTTTTTTTTTTTTTTTTTGAA144770.10635685136995514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)