FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IL12B_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IL12B_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9372535
Sequences flagged as poor quality0
Sequence length25
%GC9

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT7681028.19524280250754No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3696633.944109037736322No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1623221.7318900382874005No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1382111.4746383982561815No Hit
TATTTTTTTTTTTTTTTTTTTTTTT839890.8961182860346747No Hit
TTTTTATTTTTTTTTTTTTTTTTTT714920.7627818941193605No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG674480.7196345492441479No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA642450.6854602303432317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG618170.6595547522628616No Hit
TTTATTTTTTTTTTTTTTTTTTTTT581310.6202270783731403No Hit
TTATTTTTTTTTTTTTTTTTTTTTT470900.5024254377284267No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT423950.4523322665639552No Hit
TTTTATTTTTTTTTTTTTTTTTTTT417710.4456745160194121No Hit
TTTTTTATTTTTTTTTTTTTTTTTT387020.4129299063700483No Hit
TATTTTTTTTTTTTTTTTTTTTTTA383350.4090142101363185No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT368880.3935754841139564No Hit
TTTTTTTTTTTTTTTTTTTTTTATT322810.34442122648781787No Hit
TTTTTTTATTTTTTTTTTTTTTTTT321650.34318356773274256No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC319000.34035615764571703No Hit
TTTTTATTTTTTTTTTTTTTTTTTA311290.3321299947132766No Hit
TTTTTTTTATTTTTTTTTTTTTTTT272970.29124457790768454No Hit
TTTATTTTTTTTTTTTTTTTTTTTA255740.2728610776060052No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC238460.25442422994419334No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA226290.24143948248792885No Hit
TTATTTTTTTTTTTTTTTTTTTTTA215100.22950034329026248No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG199590.2129519921771431No Hit
TTTTTTTTTTTTTTTTTTTTTAATT197890.21113818193263614No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA196940.21012458209011756No Hit
TTTTATTTTTTTTTTTTTTTTTTTA184860.19723585988209164No Hit
TATTTTTTTTTTTTTTTTTTTTTAA176550.1883695286280606No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC167250.1784469196434049No Hit
TTTTTTATTTTTTTTTTTTTTTTTA161320.17211992273168358No Hit
TATTTTTTTTTTTTTTTTTTTTTAT145050.15476069174454937No Hit
TTTTTTTATTTTTTTTTTTTTTTTA142210.1517305616890201No Hit
TATTTATTTTTTTTTTTTTTTTTTT139010.14831633064053643No Hit
AATTTTTTTTTTTTTTTTTTTTTTT137150.14633180884360528No Hit
TATATTTTTTTTTTTTTTTTTTTTT136860.14602239415483645No Hit
TTTTTTTTTATTTTTTTTTTTTTTT135510.1445820154312574No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC135140.14418724496627647No Hit
TTTTTATTTTTTTTTTTTTTTTTAA133650.14259749363432625No Hit
TTTTTATTTTTTTTTTTTTTTTTAT133280.14220272316934532No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA130040.13874581423275562No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC129270.13792426488671422No Hit
TTTATATTTTTTTTTTTTTTTTTTT122730.1309464301813757No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC121640.12978345773048594No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT116920.12474746693397251No Hit
TTTTTTTTATTTTTTTTTTTTTTTA116750.12456608590952181No Hit
TTTATTTTTTTTTTTTTTTTTTTAA106380.11350184341802937No Hit
TTTATTTTTTTTTTTTTTTTTTTAT106330.11344849605789682No Hit
TTTTTTTTTTATTTTTTTTTTTTTT105440.1124989130475373No Hit
TTATTATTTTTTTTTTTTTTTTTTT100020.10671605920916807No Hit
GCCCCCCCCCCCCCCCCCCCCCCGC99440.1060972298316304No Hit
GCCCCCCCCCCCCCCCCCCCCCGGC97650.10418739433888484No Hit
TTATTTTTTTTTTTTTTTTTTTTAA94140.1004424096575793No Hit
TTTTTTTTTTTATTTTTTTTTTTTT94100.10039973176947324No Hit
TATTATTTTTTTTTTTTTTTTTTTT93950.1002396896890756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)