FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IFNL2_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IFNL2_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11459780
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT10819589.441350532034647No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5574564.864456385724682No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2410392.1033475337222876No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2193601.9141728724286156No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1121930.9790153039587147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG995760.868917204344237No Hit
TTTTTATTTTTTTTTTTTTTTTTTT974690.8505311620292885No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA917240.8003993095853498No Hit
TTTATTTTTTTTTTTTTTTTTTTTT863900.7538539134259121No Hit
TTATTTTTTTTTTTTTTTTTTTTTT690360.6024199417440823No Hit
TTTTATTTTTTTTTTTTTTTTTTTT614320.5360661373953077No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT570870.4981509243632949No Hit
TATTTTTTTTTTTTTTTTTTTTTTA563570.4917808195270764No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT555620.48484351357530425No Hit
TTTTTTATTTTTTTTTTTTTTTTTT549160.47920640710380125No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC520280.45400522523119985No Hit
TTTTTTTTTTTTTTTTTTTTTTATT499750.43609039615071143No Hit
TTTTTATTTTTTTTTTTTTTTTTTA466850.40738129353268565No Hit
TTTTTTTATTTTTTTTTTTTTTTTT465720.406395236208723No Hit
TTTATTTTTTTTTTTTTTTTTTTTA414550.36174341915813396No Hit
TTTTTTTTATTTTTTTTTTTTTTTT388290.3388284940897644No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC343960.30014537800900193No Hit
TTATTTTTTTTTTTTTTTTTTTTTA340140.29681198068374787No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA301960.26349545977322425No Hit
TTTTATTTTTTTTTTTTTTTTTTTA293500.2561131191000176No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG291210.2541148259390669No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA285070.24875695693983654No Hit
TTTTTTTTTTTTTTTTTTTTTAATT284150.24795414920705283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG272640.23791032637624807No Hit
TTTTTTATTTTTTTTTTTTTTTTTA260140.22700261261559992No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC251800.21972498599449553No Hit
TATTTTTTTTTTTTTTTTTTTTTAA251570.2195242840612996No Hit
TTTTTTTATTTTTTTTTTTTTTTTA224610.19599852702233378No Hit
TATTTTTTTTTTTTTTTTTTTTTAT219060.191155502112606No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC213620.18640846508397194No Hit
TTTTTATTTTTTTTTTTTTTTTTAT203610.17767356790444494No Hit
TTTTTATTTTTTTTTTTTTTTTTAA198460.17317958983505793No Hit
TATATTTTTTTTTTTTTTTTTTTTT193320.1686943379366794No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC188960.16488972737696536No Hit
TTTTTTTTTATTTTTTTTTTTTTTT185630.1619839124311287No Hit
TTTTTTTTATTTTTTTTTTTTTTTA184810.16126836640843017No Hit
TATTTATTTTTTTTTTTTTTTTTTT179340.15649515086677057No Hit
TTTATTTTTTTTTTTTTTTTTTTAT177820.15516877287347575No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA176690.15418271554951318No Hit
TTTATATTTTTTTTTTTTTTTTTTT175660.15328391993563575No Hit
TTTATTTTTTTTTTTTTTTTTTTAA173640.15152123339191503No Hit
AATTTTTTTTTTTTTTTTTTTTTTT164960.14394691695652098No Hit
TTTTTTTTTTATTTTTTTTTTTTTT153630.1340601652038695No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT151450.13215785992401252No Hit
TTATTTTTTTTTTTTTTTTTTTTAA148780.12982797226473805No Hit
TTATTTTTTTTTTTTTTTTTTTTAT143350.12508966140711253No Hit
TTATTATTTTTTTTTTTTTTTTTTT141420.12340551040246847No Hit
TTTTTTTTTTTATTTTTTTTTTTTT132750.11583992013808293No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA132170.11533380221958886No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA130000.11344022311074034No Hit
TATTATTTTTTTTTTTTTTTTTTTT129140.11268977240400777No Hit
TTTTATTTTTTTTTTTTTTTTTTAT127490.1112499541876022No Hit
TTTTATTTTTTTTTTTTTTTTTTAA122900.10724464169469222No Hit
TAATTTTTTTTTTTTTTTTTTTTTT122350.1067647022892237No Hit
TTTTTTTTTTTTTTTTTTTTTTACC118930.10378035180431038No Hit
TTTTTTATTTTTTTTTTTTTTTTAT115670.10093562005553335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)