FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IFNL2_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IFNL2_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10156453
Sequences flagged as poor quality0
Sequence length25
%GC10

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8407318.2778013150851No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3893963.833976290738509No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1650311.6248881376204862No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1640741.6154655567253646No Hit
TATTTTTTTTTTTTTTTTTTTTTTT899810.8859490611535346No Hit
TTTTTATTTTTTTTTTTTTTTTTTT806290.7938696708388253No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG747520.7360049812665899No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG664340.654106310539713No Hit
TTTATTTTTTTTTTTTTTTTTTTTT656550.64643630999917No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA637960.6281326758465776No Hit
TTATTTTTTTTTTTTTTTTTTTTTT523120.515061705105119No Hit
TTTTATTTTTTTTTTTTTTTTTTTT473430.4661371445326435No Hit
TTTTTTATTTTTTTTTTTTTTTTTT448130.4412268732007129No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT440190.43340918330444694No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT424940.41839409880595124No Hit
TATTTTTTTTTTTTTTTTTTTTTTA397000.39088449481329746No Hit
TTTTTTTTTTTTTTTTTTTTTTATT386900.38094007819462167No Hit
TTTTTTTATTTTTTTTTTTTTTTTT374470.3687015535837167No Hit
TTTTTATTTTTTTTTTTTTTTTTTA335630.3304598564085316No Hit
TTTTTTTTATTTTTTTTTTTTTTTT313900.30906459174280626No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC311970.3071643220325048No Hit
TTTATTTTTTTTTTTTTTTTTTTTA274250.27002537204671745No Hit
TTTTTTTTTTTTTTTTTTTTTAATT230330.2267819286910499No Hit
TTATTTTTTTTTTTTTTTTTTTTTA228730.2252065755633389No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC222610.2191808498498442No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA214620.21131393016833733No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG202320.19920340299905884No Hit
TTTTATTTTTTTTTTTTTTTTTTTA200750.1976575877424924No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA194740.19174016755652787No Hit
TTTTTTATTTTTTTTTTTTTTTTTA181270.17847766341261068No Hit
TATTTTTTTTTTTTTTTTTTTTTAA170260.1676372647025492No Hit
TATTTTTTTTTTTTTTTTTTTTTAT166180.1636201142268861No Hit
TTTTTATTTTTTTTTTTTTTTTTAT160100.1576337723415842No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC157520.1550935154231502No Hit
TTTTTTTATTTTTTTTTTTTTTTTA157470.15504428563790923No Hit
TTTTTTTTTATTTTTTTTTTTTTTT156790.15437476055863203No Hit
TATTTATTTTTTTTTTTTTTTTTTT152000.14965854713254717No Hit
TATATTTTTTTTTTTTTTTTTTTTT151220.14889056248278804No Hit
TTTATATTTTTTTTTTTTTTTTTTT141690.13950736541585926No Hit
TTTTTATTTTTTTTTTTTTTTTTAA138460.1363271212892926No Hit
AATTTTTTTTTTTTTTTTTTTTTTT137820.1356969800382082No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT132120.1300847845207377No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC130710.12869650457694234No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC130610.1285980450064604No Hit
TTTTTTTTATTTTTTTTTTTTTTTA130010.1280072875835688No Hit
TTTATTTTTTTTTTTTTTTTTTTAT127240.1252799574812191No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC122130.120248673429592No Hit
TTTTTTTTTTATTTTTTTTTTTTTT120920.11905731262676054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA118380.11655643953651929No Hit
GCCCCCCCCCCCCCCCCCCCCCGGC115440.11366172816435029No Hit
TTATTATTTTTTTTTTTTTTTTTTT115390.11361249837910932No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA112000.11027471893977159No Hit
TTTATTTTTTTTTTTTTTTTTTTAA109770.10807907051802434No Hit
TTTTTTTTTTTATTTTTTTTTTTTT108960.10728154799712064No Hit
GCCCCCCCCCCCCCCCCCCCCCCGC108480.10680894205880734No Hit
TATTATTTTTTTTTTTTTTTTTTTT103060.10147243333868625No Hit
TTATTTTTTTTTTTTTTTTTTTTAT102800.1012164384554332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)