FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IFNL2_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IFNL2_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10835239
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8578837.917527246053363No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4054013.741504917427294No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1688211.5580736151736017No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1685081.5551848925529008No Hit
TATTTTTTTTTTTTTTTTTTTTTTT932430.8605532374505075No Hit
TTTTTATTTTTTTTTTTTTTTTTTT858320.7921560382747441No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG707670.653118957505229No Hit
TTTATTTTTTTTTTTTTTTTTTTTT683690.6309874659894443No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA655480.6049520458201245No Hit
TTATTTTTTTTTTTTTTTTTTTTTT538000.4965280415134359No Hit
TTTTATTTTTTTTTTTTTTTTTTTT517230.47735910578437635No Hit
TTTTTTATTTTTTTTTTTTTTTTTT492160.45422163738151045No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT457840.42254720915708455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG449200.4145732272264599No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT442170.4080851377620743No Hit
TATTTTTTTTTTTTTTTTTTTTTTA416300.38420933769896537No Hit
TTTTTTTATTTTTTTTTTTTTTTTT408220.3767521879305108No Hit
TTTTTTTTTTTTTTTTTTTTTTATT394510.3640990291030959No Hit
TTTTTATTTTTTTTTTTTTTTTTTA364620.33651311244726584No Hit
TTTTTTTTATTTTTTTTTTTTTTTT343840.3173349475724532No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC333070.30739515759643143No Hit
TTTATTTTTTTTTTTTTTTTTTTTA294330.2716414469491628No Hit
TTATTTTTTTTTTTTTTTTTTTTTA244000.22519115637412335No Hit
TTTTTTTTTTTTTTTTTTTTTAATT233690.21567590710274134No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC229280.21160585382565167No Hit
TTTTATTTTTTTTTTTTTTTTTTTA226910.2094185462821817No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA213880.1973929693659734No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA208260.1922061894527661No Hit
TTTTTTATTTTTTTTTTTTTTTTTA207510.19151400352128828No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG205650.18979738241122324No Hit
TATTTTTTTTTTTTTTTTTTTTTAA181530.16753668285489598No Hit
TTTTTTTATTTTTTTTTTTTTTTTA177050.163402025557535No Hit
TTTTTATTTTTTTTTTTTTTTTTAT174450.16100244766174518No Hit
TATTTTTTTTTTTTTTTTTTTTTAT173050.15971036725631987No Hit
TTTTTTTTTATTTTTTTTTTTTTTT171290.1580860376037852No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC164400.15172715617994215No Hit
TATTTATTTTTTTTTTTTTTTTTTT159920.1475924988825812No Hit
TATATTTTTTTTTTTTTTTTTTTTT155670.1436701119375401No Hit
TTTATATTTTTTTTTTTTTTTTTTT153340.1415197209770823No Hit
TTTTTATTTTTTTTTTTTTTTTTAA150620.13900939333225598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA148680.13721893905616664No Hit
TTTTTTTTATTTTTTTTTTTTTTTA144990.13381338427329567No Hit
AATTTTTTTTTTTTTTTTTTTTTTT144660.13350882246344545No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT138230.1275744817442421No Hit
TTTATTTTTTTTTTTTTTTTTTTAT135280.12485188374709594No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC135060.1246488425405291No Hit
TTTTTTTTTTATTTTTTTTTTTTTT126920.1171363178975563No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC125710.11601959126143872No Hit
TTATTATTTTTTTTTTTTTTTTTTT121130.11179264250654738No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA120410.11112814401232865No Hit
TTTTTTTTTTTATTTTTTTTTTTTT115980.1070396324437329No Hit
TTTATTTTTTTTTTTTTTTTTTTAA115090.10621823847171254No Hit
TATTATTTTTTTTTTTTTTTTTTTT113270.10453853394465965No Hit
TTATTTTTTTTTTTTTTTTTTTTAT108640.10026543946100312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)