FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IFNL2_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IFNL2_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10018662
Sequences flagged as poor quality0
Sequence length25
%GC11

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8050488.035484179424358No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3753623.746628042746626No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1581091.5781448660509758No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1492161.4893805180771644No Hit
TATTTTTTTTTTTTTTTTTTTTTTT895890.8942212043883704No Hit
TTTTTATTTTTTTTTTTTTTTTTTT771610.7701727037003544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG681430.6801606841312743No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG669890.6686421799637516No Hit
TTTATTTTTTTTTTTTTTTTTTTTT621420.6202624661856044No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA613000.611858150319873No Hit
TTATTTTTTTTTTTTTTTTTTTTTT488870.48795937022328933No Hit
TTTTATTTTTTTTTTTTTTTTTTTT455960.4551106724630495No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT442160.44133637805128073No Hit
TTTTTTATTTTTTTTTTTTTTTTTT432370.4315646141171346No Hit
TATTTTTTTTTTTTTTTTTTTTTTA405750.40499419982428797No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT389580.38885432006788934No Hit
TTTTTTTATTTTTTTTTTTTTTTTT356650.3559856595621252No Hit
TTTTTTTTTTTTTTTTTTTTTTATT349220.3485694995998468No Hit
TTTTTATTTTTTTTTTTTTTTTTTA328000.3273890265985617No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC304420.30385294962540904No Hit
TTTTTTTTATTTTTTTTTTTTTTTT295480.2949296023760458No Hit
TTTATTTTTTTTTTTTTTTTTTTTA263760.263268687974502No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC232570.23213678632935217No Hit
TTATTTTTTTTTTTTTTTTTTTTTA219370.21896137428331247No Hit
TTTTTTTTTTTTTTTTTTTTTAATT213310.2129126623894488No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA211040.21064689077244045No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA203000.20262186707167085No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG196920.19655319243228286No Hit
TTTTATTTTTTTTTTTTTTTTTTTA196800.19643341595913705No Hit
TTTTTTATTTTTTTTTTTTTTTTTA180820.18048318228521934No Hit
TATTTTTTTTTTTTTTTTTTTTTAA176160.17583186257805683No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC174140.17381562528010228No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC158300.15800513082485465No Hit
TATTTTTTTTTTTTTTTTTTTTTAT157710.15741622983188772No Hit
TTTTTTTATTTTTTTTTTTTTTTTA154660.15437191113943158No Hit
TATATTTTTTTTTTTTTTTTTTTTT150930.15064885909914916No Hit
TATTTATTTTTTTTTTTTTTTTTTT149440.14916163455758863No Hit
TTTTTTTTTATTTTTTTTTTTTTTT146190.14591768840988947No Hit
AATTTTTTTTTTTTTTTTTTTTTTT146070.14579791193674366No Hit
TTTTTATTTTTTTTTTTTTTTTTAT145330.14505929035234447No Hit
GCCCCCCCCCCCCCCCCCCCCCGGC140020.13975918141564211No Hit
GCCCCCCCCCCCCCCCCCCCCCCGC136420.13616588722126766No Hit
TTTTTATTTTTTTTTTTTTTTTTAA135070.13481840189837727No Hit
TTTATATTTTTTTTTTTTTTTTTTT134240.1339899479591187No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC128670.12843032333060045No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC126530.12629430955950005No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA126440.1262044772046407No Hit
TTTTTTTTATTTTTTTTTTTTTTTA124920.1246873085447937No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT120140.11991621236448541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA118630.11840902507740056No Hit
TTTATTTTTTTTTTTTTTTTTTTAT115870.1156541661950468No Hit
TTTTTTTTTTATTTTTTTTTTTTTT109170.10896664644440546No Hit
TTTATTTTTTTTTTTTTTTTTTTAA106340.10614191795271664No Hit
TTATTATTTTTTTTTTTTTTTTTTT105930.10573268166946845No Hit
TATTATTTTTTTTTTTTTTTTTTTT102540.10234899630309915No Hit
TTTTTTTTTTTATTTTTTTTTTTTT101700.10151056099107846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)