FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IFNL2_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IFNL2_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12635854
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT11581979.165957441420264No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5484384.340331884176566No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2385741.8880718311560105No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2072531.6401978053877484No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1226890.970959303581697No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1064860.842728952075578No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1011810.8007452444448946No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA903300.7148705580168938No Hit
TTTATTTTTTTTTTTTTTTTTTTTT878850.695520856762036No Hit
TTATTTTTTTTTTTTTTTTTTTTTT699930.5539237791129907No Hit
TTTTATTTTTTTTTTTTTTTTTTTT653120.516878400146124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG612530.48475552186658694No Hit
TTTTTTATTTTTTTTTTTTTTTTTT611540.4839720370305007No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT594380.4703916332050054No Hit
TATTTTTTTTTTTTTTTTTTTTTTA571210.45205492244528944No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT526970.4170434384569496No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC525830.41614124379721384No Hit
TTTTTTTATTTTTTTTTTTTTTTTT513450.4063437263520138No Hit
TTTTTTTTTTTTTTTTTTTTTTATT474970.3758906995918123No Hit
TTTTTATTTTTTTTTTTTTTTTTTA462060.36567374076971765No Hit
TTTTTTTTATTTTTTTTTTTTTTTT433650.3431901001705148No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC382950.30306617977700595No Hit
TTTATTTTTTTTTTTTTTTTTTTTA380680.30126970444577783No Hit
TTATTTTTTTTTTTTTTTTTTTTTA318670.2521950633491017No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA298630.2363354309095373No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG290950.23025748793868622No Hit
TTTTATTTTTTTTTTTTTTTTTTTA286320.22659331138204036No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA284010.22476518009783905No Hit
TTTTTTTTTTTTTTTTTTTTTAATT266380.21081281882490885No Hit
TATTTTTTTTTTTTTTTTTTTTTAA258850.20485358567770726No Hit
TTTTTTATTTTTTTTTTTTTTTTTA256090.20266932492255768No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC247340.195744585209674No Hit
TTTTTTTATTTTTTTTTTTTTTTTA226020.1788719622749677No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC216490.171329931479107No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA211260.16719091562786337No Hit
TATTTTTTTTTTTTTTTTTTTTTAT209490.16579013970880005No Hit
TTTTTTTTTATTTTTTTTTTTTTTT205780.16285405007053738No Hit
TTTTTATTTTTTTTTTTTTTTTTAA199450.15784449551253124No Hit
TTTTTATTTTTTTTTTTTTTTTTAT199430.15782866753604466No Hit
TATATTTTTTTTTTTTTTTTTTTTT197140.15601636422832996No Hit
TATTTATTTTTTTTTTTTTTTTTTT196890.15581851452224757No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC190500.15076147603478166No Hit
TTTTTTTTATTTTTTTTTTTTTTTA187690.1485376453384156No Hit
AATTTTTTTTTTTTTTTTTTTTTTT184270.14583106135920848No Hit
TTTATATTTTTTTTTTTTTTTTTTT181100.14332232708608378No Hit
TTTATTTTTTTTTTTTTTTTTTTAA163840.1296627833781555No Hit
TTTATTTTTTTTTTTTTTTTTTTAT160890.12732815684638332No Hit
TTTTTTTTTTATTTTTTTTTTTTTT153160.12121064393431578No Hit
TTATTATTTTTTTTTTTTTTTTTTT145450.11510895899873488No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT143200.11332831164399336No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC142350.11265562264331323No Hit
TTTTTTTTTTTATTTTTTTTTTTTT140420.11112822291235717No Hit
TATTATTTTTTTTTTTTTTTTTTTT139030.11002817854653907No Hit
TTATTTTTTTTTTTTTTTTTTTTAA137810.109062671980857No Hit
GCCCCCCCCCCCCCCCCCCCCCGGC136260.10783600380314619No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA134560.10649062580178592No Hit
TTATTTTTTTTTTTTTTTTTTTTAT132480.10484451624718046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)