FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_IFNL2_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_IFNL2_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10246727
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT8818088.605752841858672No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4686934.574075214456284No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2122012.0709149370330646No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1596531.558087767928237No Hit
TATTTTTTTTTTTTTTTTTTTTTTT956220.9331955462461331No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG923120.9008925484205834No Hit
TTTTTATTTTTTTTTTTTTTTTTTT825580.8057011765805804No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA822680.8028710045656531No Hit
TTTATTTTTTTTTTTTTTTTTTTTT704250.6872926350043287No Hit
TTATTTTTTTTTTTTTTTTTTTTTT550540.537283758999337No Hit
TTTTATTTTTTTTTTTTTTTTTTTT507930.4956997488075949No Hit
TATTTTTTTTTTTTTTTTTTTTTTA489190.47741098206285776No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC484970.47329259382044625No Hit
TTTTTTATTTTTTTTTTTTTTTTTT458010.4469817532954669No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT443970.4332798170576809No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT429780.41943149261222634No Hit
TTTTTATTTTTTTTTTTTTTTTTTA409550.3996886030046472No Hit
TTTTTTTATTTTTTTTTTTTTTTTT381610.3724213595228994No Hit
TTTTTTTTTTTTTTTTTTTTTTATT352300.34381710374444446No Hit
TTTATTTTTTTTTTTTTTTTTTTTA345400.3370832461916864No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC339220.33105205203573784No Hit
TTTTTTTTATTTTTTTTTTTTTTTT332040.3240449364953316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG294060.28697944231362854No Hit
TTATTTTTTTTTTTTTTTTTTTTTA278680.27196977142066925No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG269230.26274731433754406No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA267750.2613029506885467No Hit
TTTTATTTTTTTTTTTTTTTTTTTA259140.252900267568366No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA235280.22961478333520546No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC231590.22601363342655661No Hit
TATTTTTTTTTTTTTTTTTTTTTAA231210.22564278330046267No Hit
TTTTTTATTTTTTTTTTTTTTTTTA223070.21769878323097705No Hit
TTTTTTTTTTTTTTTTTTTTTAATT208340.20332346123791528No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC199420.19461824248855267No Hit
TTTTTTTATTTTTTTTTTTTTTTTA194400.1897191171385751No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA192200.18757209009276815No Hit
TTTTTATTTTTTTTTTTTTTTTTAA184610.18016484678473427No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC178390.17409461577340746No Hit
TTTTTTTTATTTTTTTTTTTTTTTA167070.1630471857013464No Hit
TATTTTTTTTTTTTTTTTTTTTTAT166420.16241283680144888No Hit
TATATTTTTTTTTTTTTTTTTTTTT160600.15673297434390512No Hit
TTTTTATTTTTTTTTTTTTTTTTAT157730.15393207997051156No Hit
TTTTTTTTTATTTTTTTTTTTTTTT156630.1528585664476081No Hit
TTTATTTTTTTTTTTTTTTTTTTAA155610.15186312663546125No Hit
TATTTATTTTTTTTTTTTTTTTTTT154270.15055539198028794No Hit
TTTATATTTTTTTTTTTTTTTTTTT145310.14181113637554704No Hit
AATTTTTTTTTTTTTTTTTTTTTTT139140.13578970143344307No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT130070.12693809447641183No Hit
TTATTTTTTTTTTTTTTTTTTTTAA129330.12621591265191315No Hit
TTTATTTTTTTTTTTTTTTTTTTAT128270.125181435984388No Hit
TTTTTTTTTTATTTTTTTTTTTTTT119770.11688610421649762No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA118690.11583210912128332No Hit
TTTTATTTTTTTTTTTTTTTTTTAA117000.11418280198154981No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA115510.11272867911870786No Hit
TTATTATTTTTTTTTTTTTTTTTTT113330.1106011705005901No Hit
TTTTTTTTTTTTTTTTTTTTTTACC112080.10938126877001798No Hit
TATTATTTTTTTTTTTTTTTTTTTT111630.10894210414701203No Hit
TTTTTTTTTTTATTTTTTTTTTTTT108990.10636567169204371No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG104310.1017983596127817No Hit
TTATTTTTTTTTTTTTTTTTTTTAT103960.10145678712822152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)