FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_DUPD1_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_DUPD1_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12591184
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT11707239.297957999819555No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5381154.273744232472499No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2356971.8719208614535374No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1979211.5719014192787588No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1177680.935321094505489No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1079680.8574888588714136No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1067760.8480219175575546No Hit
TTTATTTTTTTTTTTTTTTTTTTTT877430.6968605970653753No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA872810.6931913630997688No Hit
TTATTTTTTTTTTTTTTTTTTTTTT707530.5619249150834426No Hit
TTTTATTTTTTTTTTTTTTTTTTTT668270.5307443684406487No Hit
TTTTTTATTTTTTTTTTTTTTTTTT624280.49580722511878156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG606570.48174182825062356No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT558120.44326252400092No Hit
TATTTTTTTTTTTTTTTTTTTTTTA530330.4211915257532572No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC528760.41994462157013984No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT522630.4150761358105799No Hit
TTTTTTTATTTTTTTTTTTTTTTTT521350.4140595515084205No Hit
TTTTTTTTTTTTTTTTTTTTTTATT465460.36967135100241566No Hit
TTTTTATTTTTTTTTTTTTTTTTTA455580.36182459092012315No Hit
TTTTTTTTATTTTTTTTTTTTTTTT450370.35768677512774016No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC432540.34352607348125486No Hit
TTTATTTTTTTTTTTTTTTTTTTTA377540.2998447167478452No Hit
TTATTTTTTTTTTTTTTTTTTTTTA310080.24626754719810304No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG305130.2423362250920962No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA286760.22774665194313734No Hit
TTTTATTTTTTTTTTTTTTTTTTTA283180.22490339272303542No Hit
TTTTTTTTTTTTTTTTTTTTTAATT267490.21244229295672273No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC261000.20728789286218038No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA259750.20629513475460293No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA258660.20542944968479532No Hit
TTTTTTATTTTTTTTTTTTTTTTTA256480.20369807954518018No Hit
TATTTTTTTTTTTTTTTTTTTTTAA242660.19272214590780343No Hit
TTTTTTTATTTTTTTTTTTTTTTTA223540.1775369178942981No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC221100.17559905406830684No Hit
TTTTTTTTTATTTTTTTTTTTTTTT213860.169848999109218No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC200810.159484604466109No Hit
TATATTTTTTTTTTTTTTTTTTTTT195110.1549576274955556No Hit
TTTTTATTTTTTTTTTTTTTTTTAA193950.15403634797172372No Hit
TTTTTATTTTTTTTTTTTTTTTTAT191000.1516934388378408No Hit
TATTTTTTTTTTTTTTTTTTTTTAT189620.15059743388707528No Hit
TATTTATTTTTTTTTTTTTTTTTTT189180.150247983033208No Hit
TTTTTTTTATTTTTTTTTTTTTTTA188620.1498032274010133No Hit
TTTATATTTTTTTTTTTTTTTTTTT179630.14266331109131597No Hit
AATTTTTTTTTTTTTTTTTTTTTTT166490.13222743786446134No Hit
TTTTTTTTTTATTTTTTTTTTTTTT159780.12689831234298538No Hit
TTTATTTTTTTTTTTTTTTTTTTAA159490.1266679924620274No Hit
TTTATTTTTTTTTTTTTTTTTTTAT152280.12094176369752044No Hit
TTATTATTTTTTTTTTTTTTTTTTT148390.11785230046673927No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT147540.11717722495358657No Hit
TTTTTTTTTTTATTTTTTTTTTTTT145870.11585090012186305No Hit
TATTATTTTTTTTTTTTTTTTTTTT136460.10837741708801968No Hit
TTATTTTTTTTTTTTTTTTTTTTAA134940.10717022322920544No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA128350.10193640248605691No Hit
TTTTTTTTTTTTTTTTTTTTTTACC128190.10180932944828701No Hit
TTTTTATATTTTTTTTTTTTTTTTT127710.10142811033497724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)