FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_DUPD1_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_DUPD1_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9768904
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT7966818.155275146526161No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA3720973.8089943354955684No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA1618571.656859356996445No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1424441.4581369619355455No Hit
TATTTTTTTTTTTTTTTTTTTTTTT852560.8727284043327685No Hit
TTTTTATTTTTTTTTTTTTTTTTTT762010.7800363275143251No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG723210.7403184635656159No Hit
TTTATTTTTTTTTTTTTTTTTTTTT637860.6529493994413293No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA607060.6214207857913232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG533860.5464891455581915No Hit
TTATTTTTTTTTTTTTTTTTTTTTT489240.5008136020171762No Hit
TTTTATTTTTTTTTTTTTTTTTTTT468510.47959320718066223No Hit
TTTTTTATTTTTTTTTTTTTTTTTT424190.4342247605258482No Hit
TATTTTTTTTTTTTTTTTTTTTTTA391510.40077167305564676No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT386230.39536676785850283No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT368810.3775346753330773No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC348750.3570001302090797No Hit
TTTTTTTATTTTTTTTTTTTTTTTT348660.35690800114321936No Hit
TTTTTTTTTTTTTTTTTTTTTTATT331760.33960820988720947No Hit
TTTTTATTTTTTTTTTTTTTTTTTA331380.33921922049802106No Hit
TTTTTTTTATTTTTTTTTTTTTTTT309700.3170263521885362No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC288790.29562169922030146No Hit
TTTATTTTTTTTTTTTTTTTTTTTA278370.284955200706241No Hit
TTATTTTTTTTTTTTTTTTTTTTTA220490.22570597479512544No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG207360.21226536774237928No Hit
TTTTATTTTTTTTTTTTTTTTTTTA206000.21087319519159978No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA202750.20754631225775172No Hit
TTTTTTTTTTTTTTTTTTTTTAATT192510.197064071875412No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA181830.18613142272664362No Hit
TTTTTTATTTTTTTTTTTTTTTTTA180700.18497469112195186No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC176550.18072651752949973No Hit
TATTTTTTTTTTTTTTTTTTTTTAA173560.17766578523035953No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA170900.1749428595060408No Hit
TTTTTTTATTTTTTTTTTTTTTTTA154280.1579296920104855No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC146960.15043652798717236No Hit
TATTTTTTTTTTTTTTTTTTTTTAT146160.1496176029573021No Hit
TTTTTTTTTATTTTTTTTTTTTTTT145930.14938216201121435No Hit
TTTTTATTTTTTTTTTTTTTTTTAT144420.1478364410173342No Hit
TATTTATTTTTTTTTTTTTTTTTTT143560.1469560966102236No Hit
TATATTTTTTTTTTTTTTTTTTTTT143130.14651592440666836No Hit
TTTTTATTTTTTTTTTTTTTTTTAA141300.14464263340084005No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC140000.14331188022730085No Hit
TTTATATTTTTTTTTTTTTTTTTTT135840.13905347007197533No Hit
TTTTTTTTATTTTTTTTTTTTTTTA132880.13602344746145523No Hit
AATTTTTTTTTTTTTTTTTTTTTTT120090.12293088354640397No Hit
TTTATTTTTTTTTTTTTTTTTTTAT118370.12117019473218285No Hit
TTTATTTTTTTTTTTTTTTTTTTAA113990.116686580193643No Hit
TTTTTTTTTTATTTTTTTTTTTTTT110850.11347229945140212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA109460.1120494172120025No Hit
GCCCCCCCCCCCCCCCCCCCCCCCC107500.11004305088882027No Hit
TTATTATTTTTTTTTTTTTTTTTTT103770.10622481293705005No Hit
TTTTTTTTTTTATTTTTTTTTTTTT102670.10509879102097841No Hit
TATTATTTTTTTTTTTTTTTTTTTT98880.1012191336919679No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT98700.1010348755602471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)