FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_DUPD1_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_DUPD1_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11731428
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT11390359.709261310728753No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5628824.798068913690644No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2497692.129058798298042No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT2070501.7649172803174513No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1101730.9391269332258614No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1034120.881495415562368No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1029580.8776254689539926No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA982100.8371529876840228No Hit
TTTATTTTTTTTTTTTTTTTTTTTT849270.7239272149988901No Hit
TTATTTTTTTTTTTTTTTTTTTTTT671880.5727180015936679No Hit
TTTTATTTTTTTTTTTTTTTTTTTT632310.5389880924982023No Hit
TTTTTTATTTTTTTTTTTTTTTTTT570680.48645399349508No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC554740.47286655980840525No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT547660.466831488886093No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT540330.4605833151769759No Hit
TATTTTTTTTTTTTTTTTTTTTTTA524460.44705555027060645No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG506570.4318059148468541No Hit
TTTTTTTTTTTTTTTTTTTTTTATT479310.4085691869736574No Hit
TTTTTTTATTTTTTTTTTTTTTTTT470450.40101682420929485No Hit
TTTTTATTTTTTTTTTTTTTTTTTA460370.39242451984532484No Hit
TTTTTTTTATTTTTTTTTTTTTTTT404210.34455310981749193No Hit
TTTATTTTTTTTTTTTTTTTTTTTA381310.3250328945461712No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC361670.30829153961478517No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA331080.2822162826213484No Hit
TTATTTTTTTTTTTTTTTTTTTTTA311850.26582441626032227No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG306650.26139187829478217No Hit
TTTTATTTTTTTTTTTTTTTTTTTA287450.2450255842681726No Hit
TTTTTTTTTTTTTTTTTTTTTAATT279600.23833415676250153No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA264320.22530931443299146No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC263900.22495130175115935No Hit
TTTTTTATTTTTTTTTTTTTTTTTA251860.21468827153863962No Hit
TATTTTTTTTTTTTTTTTTTTTTAA243940.20793717525266317No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC225210.19197151446524668No Hit
TTTTTTTATTTTTTTTTTTTTTTTA210980.17984170384031678No Hit
TTTTTATTTTTTTTTTTTTTTTTAA203940.1738407293638933No Hit
TTTTTTTTTATTTTTTTTTTTTTTT199030.1696553906310468No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC196670.16764370032360937No Hit
TTTTTATTTTTTTTTTTTTTTTTAT193630.16505237043606286No Hit
TATTTTTTTTTTTTTTTTTTTTTAT190520.162401371768211No Hit
TTTTTTTTATTTTTTTTTTTTTTTA180770.15409036308282334No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA177520.15132002685436077No Hit
TTTATATTTTTTTTTTTTTTTTTTT176820.1507233390513073No Hit
TATTTATTTTTTTTTTTTTTTTTTT175900.1499391207958656No Hit
TATATTTTTTTTTTTTTTTTTTTTT175330.14945324644195063No Hit
TTTATTTTTTTTTTTTTTTTTTTAA163710.13954822891126298No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT163370.13925840912120843No Hit
TTTATTTTTTTTTTTTTTTTTTTAT155940.13292499429736943No Hit
TTTTTTTTTTATTTTTTTTTTTTTT154320.13154408823887423No Hit
AATTTTTTTTTTTTTTTTTTTTTTT153490.13083658698668227No Hit
TTTTTTTTTTTATTTTTTTTTTTTT140050.11938018116805559No Hit
TTATTTTTTTTTTTTTTTTTTTTAA138210.11781174465717217No Hit
TTATTATTTTTTTTTTTTTTTTTTT137910.1175560213130064No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA135160.11521189065815346No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA128820.10980760398478344No Hit
TATTATTTTTTTTTTTTTTTTTTTT128130.10921944029320216No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA125870.10729299110048666No Hit
TTATTTTTTTTTTTTTTTTTTTTAT125390.10688383374982141No Hit
TTTTTTTTTTTTTTTTTTTTTTACC124640.10624452538940699No Hit
TTTTATTTTTTTTTTTTTTTTTTAA124540.10615928427468507No Hit
TTTTATTTTTTTTTTTTTTTTTTAT121550.1036105749444995No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG120460.10268144679403052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)