FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_DUPD1_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_DUPD1_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9639153
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT96642310.026015771302728No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA4853225.034902962947055No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2191132.2731561580151287No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1639301.7006680981202393No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG987711.0246854676961763No Hit
TATTTTTTTTTTTTTTTTTTTTTTT935420.9704379627546114No Hit
TTTTTATTTTTTTTTTTTTTTTTTT851090.882951022771399No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA831550.8626795321124169No Hit
TTTATTTTTTTTTTTTTTTTTTTTT715430.7422125159752108No Hit
TTATTTTTTTTTTTTTTTTTTTTTT574100.5955917496070453No Hit
TTTTATTTTTTTTTTTTTTTTTTTT531680.5515837335500329No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC501350.5201183132999342No Hit
TTTTTTATTTTTTTTTTTTTTTTTT478500.4964129109684222No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT462410.4797205729590556No Hit
TATTTTTTTTTTTTTTTTTTTTTTA462020.47931597309431645No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT443520.460123415407972No Hit
TTTTTATTTTTTTTTTTTTTTTTTA401780.4168208555253765No Hit
TTTTTTTATTTTTTTTTTTTTTTTT387030.4015186811538317No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC378500.39266935590710095No Hit
TTTTTTTTTTTTTTTTTTTTTTATT366450.3801682575222118No Hit
TTTATTTTTTTTTTTTTTTTTTTTA338440.35110968774953566No Hit
TTTTTTTTATTTTTTTTTTTTTTTT333130.3456009049757795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG303140.3144882128128893No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG283510.2941233529543519No Hit
TTATTTTTTTTTTTTTTTTTTTTTA278320.2887390624466693No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA273800.2840498537578976No Hit
TTTTATTTTTTTTTTTTTTTTTTTA256300.26589473162216637No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC245340.25452443798744556No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA236910.24577885629577617No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA228940.2375104949573889No Hit
TTTTTTATTTTTTTTTTTTTTTTTA220740.22900352344236058No Hit
TATTTTTTTTTTTTTTTTTTTTTAA214600.22263366916159544No Hit
TTTTTTTTTTTTTTTTTTTTTAATT212920.22089077743656524No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC208300.21609782519273218No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC190280.1974032365706821No Hit
TTTTTTTATTTTTTTTTTTTTTTTA185750.19270365352640423No Hit
TTTTTATTTTTTTTTTTTTTTTTAA179930.18666577862183537No Hit
TTTTTTTTTATTTTTTTTTTTTTTT162890.16898787683938618No Hit
TTTTTTTTATTTTTTTTTTTTTTTA156660.16252465335906588No Hit
TATTTTTTTTTTTTTTTTTTTTTAT154250.16002443368208805No Hit
TTTTTATTTTTTTTTTTTTTTTTAT154230.16000368497107578No Hit
TTTATTTTTTTTTTTTTTTTTTTAA148810.15438078428675214No Hit
TATATTTTTTTTTTTTTTTTTTTTT148250.15379982037840878No Hit
TATTTATTTTTTTTTTTTTTTTTTT146830.152326661896538No Hit
TTTATATTTTTTTTTTTTTTTTTTT144590.1500028062631644No Hit
AATTTTTTTTTTTTTTTTTTTTTTT134720.139763317378612No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT132090.13703486188049924No Hit
TTTTTTTTTTATTTTTTTTTTTTTT127990.13278137612298507No Hit
TTATTTTTTTTTTTTTTTTTTTTAA127360.13212779172609876No Hit
TTTATTTTTTTTTTTTTTTTTTTAT126900.1316505713728167No Hit
TTTTTTTTTTTTTTTTTTTTTTACC119860.12434702509649966No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA119550.12402542007580955No Hit
TTTTTTTTTTTATTTTTTTTTTTTT115970.12031140080461426No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA113910.11817428357035105No Hit
TTATTATTTTTTTTTTTTTTTTTTT113800.11806016565978358No Hit
TTTTATTTTTTTTTTTTTTTTTTAA113410.11765556579504445No Hit
TTTTTTTTTTTTTTTTTTTTAAAAA105620.1095739428557675No Hit
TATTATTTTTTTTTTTTTTTTTTTT105320.10926271219058356No Hit
TTTTTTTTTTTTTTTTTTTTTTTAG102950.10680398993563023No Hit
TTATTTTTTTTTTTTTTTTTTTTAT100550.10431414461415853No Hit
TAATTTTTTTTTTTTTTTTTTTTTT96580.10019552547822408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)