FastQCFastQC Report
Fri 28 May 2021
H2Y77BGXJ_n02_DUPD1_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2Y77BGXJ_n02_DUPD1_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11919939
Sequences flagged as poor quality0
Sequence length25
%GC4

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT10882029.129258127914916No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA5444534.567582099203696No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA2423782.0333828889560595No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT1963241.6470218513702124No Hit
TATTTTTTTTTTTTTTTTTTTTTTT1173710.9846610792219659No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG1017440.8535614150374427No Hit
TTTTTATTTTTTTTTTTTTTTTTTT1006950.8447610344314682No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA938550.7873781904420819No Hit
TTTATTTTTTTTTTTTTTTTTTTTT854250.7166563520165665No Hit
TTATTTTTTTTTTTTTTTTTTTTTT707610.5936355882358123No Hit
TTTTATTTTTTTTTTTTTTTTTTTT622370.5221251551706766No Hit
TATTTTTTTTTTTTTTTTTTTTTTA572660.48042192162224995No Hit
TTTTTTATTTTTTTTTTTTTTTTTT569340.4776366724695487No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC551370.4625610919653196No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT550540.4618647796771443No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT516910.43365154804902944No Hit
TTTTTTTATTTTTTTTTTTTTTTTT485300.4071329559656303No Hit
TTTTTATTTTTTTTTTTTTTTTTTA464750.38989293485478405No Hit
TTTTTTTTTTTTTTTTTTTTTTATT439680.3688609480300193No Hit
TTTTTTTTATTTTTTTTTTTTTTTT402770.33789602446791045No Hit
TTTATTTTTTTTTTTTTTTTTTTTA401620.3369312544300772No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC377390.3166039691981645No Hit
TTATTTTTTTTTTTTTTTTTTTTTA343100.28783704346138017No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA312140.2618637561819738No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG310790.2607312000506043No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG299410.25118417132839355No Hit
TTTTATTTTTTTTTTTTTTTTTTTA293750.2464358248813186No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA277510.23281159408617777No Hit
TATTTTTTTTTTTTTTTTTTTTTAA265820.2230044969189859No Hit
TTTTTTATTTTTTTTTTTTTTTTTA257480.21600781681852566No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC255990.21475781042168085No Hit
TTTTTTTTTTTTTTTTTTTTTAATT255460.21431317727380989No Hit
TTTTTTTATTTTTTTTTTTTTTTTA227380.190756009741325No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC226960.19040365894489897No Hit
TTTTTATTTTTTTTTTTTTTTTTAA207080.17372572124739902No Hit
TATTTTTTTTTTTTTTTTTTTTTAT204380.17146060898466006No Hit
TTTTTTTTTATTTTTTTTTTTTTTT200850.16849918443374584No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC199310.167207231513517No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA196180.16458137914967516No Hit
TATATTTTTTTTTTTTTTTTTTTTT193070.161972305395187No Hit
TTTTTATTTTTTTTTTTTTTTTTAT190980.16021894071773354No Hit
TATTTATTTTTTTTTTTTTTTTTTT188280.1579538284549946No Hit
TTTTTTTTATTTTTTTTTTTTTTTA186350.15633469265237013No Hit
TTTATATTTTTTTTTTTTTTTTTTT176050.14769370883525496No Hit
TTTATTTTTTTTTTTTTTTTTTTAA173140.145252421174303No Hit
AATTTTTTTTTTTTTTTTTTTTTTT170020.142634958115138No Hit
TTTATTTTTTTTTTTTTTTTTTTAT157410.13205604491767953No Hit
TTATTTTTTTTTTTTTTTTTTTTAA155190.13019361927942752No Hit
TTTTTTTTTTATTTTTTTTTTTTTT153530.12880099470307693No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT147110.12341506110056435No Hit
TTATTATTTTTTTTTTTTTTTTTTT145490.12205599374292099No Hit
TTTTTTTTTTTATTTTTTTTTTTTT137300.11518515321261291No Hit
TTATTTTTTTTTTTTTTTTTTTTAT133590.1120727211775161No Hit
TATTATTTTTTTTTTTTTTTTTTTT133530.11202238534945522No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA133330.11185459925591901No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA133270.11180426342785815No Hit
TTTTATTTTTTTTTTTTTTTTTTAA128440.1077522292689585No Hit
TAATTTTTTTTTTTTTTTTTTTTTT128370.10769350413622084No Hit
TTTTTTTTTTTTTTTTTTTTTTACC126360.10600725389618186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)